[Objective]The detection method of protease activity in polyglucose and stevioside glycosides was established.[Method]Single factor experiment and orthogonal experiment were used to optimize the assay conditions of protease activity,and the accuracy of the recovery method was investigated.[Result]The optimal conditions for the detection of protease activity in polyglucose and stevioside glycosides were to dissolve samples with boric acid buffer solution,sample size to be tested was 5 g,reaction time was 20 min,reaction temperature was 40 ℃,pH value was 10.5,and filtrate standing time was 20 min.The experimental results showed that the accuracy of the method was 64％～102％ and the relative standard deviation was less than 1.3％.[Conclusion]This method was suitable for the detection of protease content in stevioside polyglucose after comprehensive verification of curve correlation and accuracy.
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