摘要
泛酸、生物素作为婴幼儿配方乳粉的关键营养强化剂,对确保婴幼儿健康成长至关重要.本研究综述婴幼儿配方乳粉中泛酸、生物素的几种常用检测方法,包括高效液相色谱法(HPLC)、超高效液相色谱-串联质谱法(UPLC-MS/MS)、酶联免疫吸附试验(ELISA)、荧光免疫分析、表面等离子共振(SPR)技术以及微生物法.详细阐述这些方法的工作原理、适用性、优势与局限性,进行全面比较和总结.还评述当前婴幼儿配方乳粉中泛酸、生物素检测的研究进展.
Abstract
Pantothenic acid and biotin,as key nutritional fortifiers in infant formula milk powder,are essential to ensure the healthy growth of infant and young children.In this study,we reviewed several commonly used detection techniques for the analysis of pantothenic acid and biotin in infant formula milk powder,including high-performance liquid chromatography(HPLC),ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),enzyme-linked immunosorbent assay(ELISA),fluorescence immunoassay,surface plasmon resonance(SPR) technology,and microbial method.This paper elaborated on the working principles,applicability,advantages,and limitations of these methods,and made a comprehensive comparison and summary.In addition,this paper reviewed the current research progress in the field of pantothenic acid and biotin detection in infant formula milk powder.
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