Cloning, sequence analysis, and expression distribution of PPARα from rainbow trout
Peroxisome proliferator-activated receptors (PPARs) belong to the nuclear receptor superfamily, which regulates the biosynthesis and metabolism of lipids, and the differentiation and formation of lipocytes. PPARa mRNA from the rainbow trout (Oncorhynchus mykiss) was cloned by RT-PCR. Sequence homology analysis showed that rainbow trout PPARa gene was similar to those reported in other vertebrate species. The mRNA sequence and the deduced amino acid sequence of rainbow trout PPARa showed identities of 66.4%-97.5% and 72.5%-93.8%, respectively, with those of other vertebrates. The DNA-binding domain and the ligand-binding domain of PPARa have been highly conserved in the evolution from fish to humans, which show 88.0%-98.8% and 74.6%-99.6% amino acid sequence identities, respectively, with those of other vertebrates. Phylogenetic analysis indicated that PPARa of rainbow trout and Atlantic salmon (Salmo salar) belong to the same branch of the phylogenetic tree. Using quantitative real-time RT-PCR, we observed that rainbow trout mRNA was expressed predominantly in lipid, muscle, ovarian, renal, and intestinal tissues.
PPARaOncorhynchus mykisscloningsequence analysisexpression distribution
国家科技期刊平台
NSTL
万方数据
维普
