首页|5-HMF对黄条鰤生长、肠道组织结构及生理特性的影响

5-HMF对黄条鰤生长、肠道组织结构及生理特性的影响

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  • 国家科技期刊平台
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为了分析在饲料中添加 5-HMF(5-羟甲基糠醛)对黄条鰤(Seriola aureovittata)幼鱼生长、肠道组织结构、血清生化特性、消化道菌群结构等方面的影响,选用 360 尾平均体重(82.33±2.75)g的黄条鰤,随机分为 4 组(M0 组、M1 组、M2 组、M3 组),每组 3 个重复,每个重复 30 尾鱼,M0 组为对照组,投喂基础饲料,M1 组、M2 组、M3组分别投喂添加 5-HMF(添加量分别为 0.25%、0.5%、1%)的基础饲料,实验持续 6 周.结果显示:体外Caco-2 细胞(人结直肠腺癌细胞)增殖实验表明,本研究所用 5-HMF浓度在安全范围内;M2 和M3 组实验鱼的增重率和特定生长率显著高于M0 组(P<0.05),且M2 组肥满度显著高于M0 组(P<0.05);M2 组实验鱼肠道肌层厚度和绒毛高度显著高于M0 组(P<0.05);M2 和M3 组血清中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性显著高于M0组(P<0.05),而丙二醛(MAD)水平显著降低(P<0.05);M2和M3组实验鱼血清免疫球蛋白M(IgM)和碱性磷酸酶(AKP)水平显著高于M0 组(P<0.05);M2 组血清溶菌酶(LZM)和酸性磷酸酶(ACP)活性显著高于M0 组(P<0.05);在M0 组幽门盲囊微生物Chao1 指数显著高于M1 组(P<0.05),而M2 组肠道微生物的Shannon指数显著高于M0 和M1 组(P<0.05).综上所述,饲料中添加 0.5%的 5-HMF实验饲料可以提升黄条鰤幼鱼生长性能,提高血清免疫酶活水平和抗氧化能力,对肠道组织和菌群结构起到一定的优化调节作用,可为开发黄条鰤健康养殖技术和绿色饲料添加剂提供理论支撑.
Effects of 5-HMF on growth,intestinal tissue structure,physiological characteristics of juvenile yellowtail kingfish(Seriola aureovittata)
This study was conducted to analyze the effects of 5-HMF(5-hydroxymethylfurfural)on the growth performance,intestinal histology,serum biochemical characteristics,and gastrointestinal microecology of juvenile yellowtail kingfish.A total of 360 6-month-old yellowtail kingfish juveniles with an initial mean body mass of(82.33±2.75)g were randomly divided into four groups(M0 group,M1 group,M2 group,and M3 group)with three replicates per group and 30 fish assigned in each replicate.The M0 group was the control group and was fed the basic diet,whereas the M1,M2,and M3 groups were fed the experimental diets(0.25%5-HMF,0.5%5-HMF,and 1%5-HMF,respectively).The experiment lasted for six weeks.The results showed that the proliferation experiment of Caco-2 cells(human colorectal adenocarcinoma cells)in vitro showed that the concentration of 5-HMF used in this study was within the safe range.The weight gain rate and specific growth rate of the M2 and M3 groups were significantly higher than those of the M0 group(P<0.05)and the plumpness of the M2 group was significantly higher than that of the M0 group(P<0.05).The muscle layer thickness and villus height of the M2 group were significantly higher than those of the M0 group(P<0.05).The activities of glutathione peroxidase(GSH-PX),superoxide dismutase(SOD),and catalase(CAT)in the serum of M2 and M3 groups were significantly higher than those in the serum of M0 group(P<0.05),while the level of malondialdehyde(MAD)was significantly lower(P<0.05).The serum levels of immunoglobulin M(IgM)and alkaline phosphatase(AKP)in M2 and M3 groups were significantly higher than those in the M0 group(P<0.05).The activities of lysozyme(LZM)and acid phosphatase(ACP)in the M2 group were significantly higher than those in the M0 group(P<0.05).In the M0 group,the Chao1 index of pyloric caecum microbiota was significantly higher than that of the M1 group(P<0.05),while the Shannon index of intestinal microbiota in the M2 group was significantly higher than that in the M0 and M1 groups(P<0.05).The results of this study showed that the addition of 0.5%5-HMF to feed could improve the growth performance and promote serum immune levels and antioxidant capacity of yellowtail kingfish.5-HMF may have played a role in optimizing and regulating the intestinal morphology and microbial community structure of juvenile yellowtail kingfish.This provides theoretical support for the development of healthy breeding technologies and green feed additives for yellowtail kingfish.

5-hydroxymethylfurfural(5-HMF)Seriola aureovittataintestinal histologygrowthantioxidantmicrobial microecology

冯元、周鹤庭、徐永江、崔爱君、姜燕、陈伟

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中国水产科学研究院黄海水产研究所深蓝渔业工程联合实验室,山东 青岛 266071

上海海洋大学水产科学国家级实验教学示范中心,上海 201306

中科国生(杭州)科技有限公司,浙江 杭州 310000

5-羟甲基糠醛(5-HMF) 黄条鰤 肠道组织结构 生长 抗氧化 菌群微生态

山东省重点研发计划青岛海洋科技中心山东省专项山东省泰山产业领军人才工程项目中国水产科学研究院基本科研业务费专项现代农业产业技术体系建设项目

2023TZXD0502022QNLM0300012023TD51CARS-47

2024

10.12264/JFSC2023-0212

中国水产科学
中国水产科学研究院 中国水产学会

中国水产科学

CSTPCD北大核心
影响因子：1.206
ISSN：1005-8737
年,卷(期)：2024.31(2)
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