Analysis of bacterial communities in sediment of intensive/semi-intensive ponds in Harbin and their correlation with environmental factors
To investigate the structural attributes of bacterial communities within the sediments of main Carp(Cyprinus carpio)ponds under two aquaculture practices of intensive aquaculture(JY)and semi-intensive aquaculture(CF)in Harbin,16S rDNA high-throughput sequencing method was used to assess these communities along with their pre-dicted functions via PICRUSt.Subsequently,the principal component analysis(PCA)method was conducted to elu-cidate the correlation between environmental variables and bacterial community composition.The pollution index eval-uation results showed that the water quality in the intensive pond was superior to that in the semi-intensive ponds.Although the concentrations of total nitrogen(TN)and total phosphorus(TP)in the water were lower than those of semi-intensive ponds,these differences were not significant(P＞0.05).Among them,the Shannon and Simpson indices were positively correlated with the concentration of ammonia(NH4+-N)in the sediment.However,the con-centration of total organic carbon(TOC)was significantly reduced in the intensive ponds(P＜0.05).In addition,the pH levels in the water and sediment of the semi-intensive ponds were significantly lower than those in the inten-sive ponds(P＜0.05).Alpha diversity indices for the bacterial communities in the intensive pond sediments were higher,though the discrepancy was not statistically significant(P＞0.05).The distribution of dominant bacterial phylum in the sediment of the two ponds remained relatively similar,with the common dominant bacterial phylum be-ing Proteobacteria,Chloroflexi,Acidobacteria,and Bacteroidetes.Nonetheless,differences were observed among the predominant bacterial genera with Smithella and Clostridium_sensu_stricto dominating in the intensive ponds,and Arenimonas and Luteimonas in the semi-intensive ponds.Furthermore,the main functions of the sediment microbiota in the two aquaculture ponds were essentially consistent,but there was a significant difference in their metabolic processes(P＜0.05).PCA highlighted the NH4+-N,pH,and TOC of water and the pH of sediment as key envi-ronmental factors influencing the sediment microbiota structure in both aquaculture settings.The results of this study offer theoretical and data support for understanding the impact of altered environmental factors on the bacterial commu-nity structure within freshwater aquaculture pond sediments and shed light on the microbial roles and functions within pond ecosystems.[Chinese Fishery Quality and Standards,2024,14(1):09-18]
bacterial community structurediversitysedimentpondintensive/semi-intensive
万方数据
维普
