Strontium labeling technique for otolith of Myxocyprinus asiaticus based on immersion method
Fish scale labeling is one of the key techniques for the proliferation and release of aquatic organisms.In or-der to investigate the safety and effectiveness of the strontium otolith labeling technique during the proliferation and re-lease of Myxocyprinus asiaticus,individuals with a mean body length of(10.47±1.91)cm and a mean body weight of(12.53±5.80)g were selected as the study subjects.The strontium concentration in the SrCl2?6H2 O solution was progressively increased to five different gradients:0,12,18,36,and 72 mg/L,respectively,to assess the im-pact of strontium labeling on Myxocyprinus asiaticus at different strontium concentrations.The results showed:(1)No significant differences were observed in the sucker region of Myxocyprinus asiaticus among the different strontium con-centrations;(2)Quantitative line analysis revealed that the Sr/Ca ratio in the experimental group exposed to 12-72 mg/L strontium concentration exhibited a distinct peak value in specific areas.As the exogenous strontium concentra-tion increased,the Sr/Ca ratio at the edge of the otolith first increased and then stabilized;(3)Following the change of time after labeling,the content of strontium in muscle showed a trend of initial increasing and then decreasing,with the strontium residue in the muscle of the experimental group,at strontium concentrations of 12-18 mg/L,being completely metabolized within 7 d.It is suggested that a strontium concentration of 12 mg/L and a duration of 7 d should be chosen as optimal conditions for labeling exogenous strontium.[Chinese Fishery Quality and Standards,2024,14(2):22-27]
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