Research on detection techniques for quinolone and sulfonamide drug residues in fishery herbal powder
A method for determining quinolone and sulfonamide antibiotic residues in Chinese herbal powder for fishery has been established using the QuEChERS technique combined with high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) . The QuEChERS method was optimized across four key aspects:extraction solvent,ex-traction solvent volume,purification agent,purification agent volume,and other relevant factors. Quantify using the in-ternal standard method,and ultimately select the method conditions that yield the best recovery results. The results showed that the 14 antibiotics tested—namely,ofloxacin,enrofloxacin,lomefloxacin,difloxacin,sulfamethoxypyrimi-dine,sulfamethoxypyrimidine,sulfadimethoxypyrimidine,sulfamethoxypyrimidine,sulfamethoxazole,sulfamethoxazole,and sulfaquinoxaline—exhibited excellent linearity within the concentration range of 2 to 200 ng/mL,with the correla-tion coefficients (R2) exceeding 099,and the quantitative limits all set at 1.0μg/kg. The average recoveries of these 14 antibiotics at three concentration levels (1 μg/kg,25μg/kg,and 50 μg/kg) ranged from 72.5％ to 123.5％,with the relative standard deviations of 09％ to 12.9％. The method is characterized by its simplicity,rapidity,accuracy,high sensitivity,and selectivity,making it highly suitable for rapidly detecting of quinolone and sulfonamide residues in Chinese herbal powder used in the fishery industry.[Chinese Fishery Quality and Standards,2024,14(4):28-35]
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