Determination of Tiletamine and Etomidate in E-Cigarette Oil by HPLC-MS/MS
Objective To establish a rapid method for the determination of tiletamine and etomidate in e-cigarette oil using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Methods The samples were diluted with methanol and filtered through filter membrane.C18 chromatography column(2.1 mm×100 mm,2 μm)was used as separation column.Gradient elution was performed with the mobile phase of acetonitrile-0.1%(volume fraction)formic acid aqueous solution.The multiple reaction monitoring(MRM)mode and positive ionization was used for the qualitative and quantitative analysis of tiletamine and etomidate.Results Tiletamine had good linear relationship in the concentration range of 0.02-10.00 ng/mL,and etomidate had good linear relationship in the concentration range of 0.05-10.00 ng/mL.The correlation coefficients were greater than 0.99.The limit of detection for tiletamine and etomidate were 0.01 ng/mL and 0.02 ng/mL,respectively,and the limit of quantification were 0.02 ng/mL and 0.05 ng/mL,respectively.In the spike recovery experiment,the recovery rate of the samples ranged from 95.81%to 102.05%.Conclusion The method is simple to operate with good reproducibility and can be applied to the qualitative and quantitative detection of trace amounts of tiletamine and etomidate in e-cigarette oil.
high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)tiletamineetomidatee-cigarette oil
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