Analysis of Allele Drop-out in the Application of STRtyper-21G(Plus)Kit in Forensic Genetics
Objective Analyze the drop-out of alleles in the STRtyper-21G(Plus)kit in forensic genetics application,explore its impact on identification results,and propose solutions.Methods Using the STRtyper-21G(Plus)kit,20 autosomal short tandem repeat(STR)loci of 2 540 blood stains were directly amplified.Capillary electrophoresis was used to analyze the genotypes of each locus.For samples containing loci that do not in accordance with Mendelian inheritance,the STRtyper-32G kit was used for validation.Results After verification by the STRtyper-32G kit,it was found that 9 individuals in 5 cases had allelic drop-out detected using the STRtyper-21G(Plus)kit,involving three loci:D7S820,D19S433,and D18S51.Conclusion In the practice of forensic genetics,using any detection system for detecting STRs may result in allele drop-out and inaccurate paternity test results.To ensure the accuracy of paternity test results,other detection systems with the same locus can be used for verification.
万方数据
维普
