首页|黄芪多糖/壳聚糖微球复合温敏凝胶的制备及理化特性研究

黄芪多糖/壳聚糖微球复合温敏凝胶的制备及理化特性研究

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目的 制备载黄芪多糖/壳聚糖微球(Astragalus polysaccharides/Chitosan Microshperes,APS-MS)的壳聚糖温敏凝胶(Thermosensitive hydrogels,HG)并考察其理化特性.方法 采用喷雾干燥法制备APS-MS,用扫描电镜和激光粒度仪观察微球的形态和粒径.以适宜的成胶温度和时间为指标,采用单因素实验优化黄芪多糖/壳聚糖微球复合温敏凝胶(APS-MS-HG)的制备工艺,扫描电镜观察微球在温敏凝胶中的存在状态,并考察APS-MS、黄芪多糖温敏凝胶(APS-HG)和APS-MS-HG的体外释药行为.结果 APS-MS为球形,表面有少许皱褶,分布均匀,无明显粘连,表面积平均粒径D[3,2]为8.078μm.最佳APS-MS-HG(APS-MS-HG J)的制备工艺为加入3.012%的APS-MS,磁力搅拌3h.电镜扫描显示APS-MS以球形不规则的分散在温敏凝胶中,而空白温敏凝胶的多孔结构消失.体外释放结果表明,pH6.8的磷酸缓冲溶液中,APS-MS-HG J没有突释现象,36h累计药物释放量为74.75%.结论 APS-MS-HG J具有良好的缓释作用,且能改善APS-MS和APS-HG J的突释现象,有望成为鼻粘膜给药的良好载体.
Preparation and characterization of thermosensitive chitosan hydrogels containing astragalus polysaccharides/chitosan microshperes
Objective To prepare thermosensitive chitosan (CTS) hydrogels containing astragalus polysaccharides (APS)/CTS microshperes (MS), and evaluate its physicochemical properties. Methods The APS/CTS MS (APS-MS) were prepared by spray drying method, and characterized by Scanning Electron Microscopy (SEM) and Laser Granularity Analyzer. Depending on the gelation temperature and gelating time, thermosensitive CTS hydrogels (HG) containing APS-MS (APS-MS-HG) were optimized by signal factor experiments, and the morphological characteristics were observed by SEM. In vitro release behaviors of APS-MS, hydrogels containing APS (APS-HG) and APS-MS-HG in pH 6.8 phosphate buffer were evaluated by dialysis tube method. Results The APS-MS were well dispersed with nearly spherical shapes and slightly wrinkled surfaces. The surface weighted mean D[3,2] of APS-MS was 8.078μm. The optimal APS-MS-HG, APS-MS-HG J, contained 3.012% APS-MS which were agitated with a magnetic stirrer for 3h. Observed by SEM, APS-MS were stayed spherical and dispersed unevenly in HG J, but the porous structure of HG J was disappeared in APS-MS-HG J. The release of APS from APS-MS-HG J was without initial burst release, and the cumulative amount of APS was about 74.75% after 36h. Conclusion Suppressing the phenomenon of sudden release at the first stage of delivery, APS-MS-HG J holds great promise for topical applications as a sustained-release nasal delivery system.

astragalus polysaccharideschitosanmicroshperesthermosensitive hydrogelsin vitro release

杨婷婷、衣君丽、张竞竞、于洪丽、王永红、张维芬

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潍坊医学院 药学院,山东 潍坊 261053

青岛滨海学院 医学院,山东 青岛 266555

黄芪多糖 壳聚糖 微球 温敏凝胶 体外释放

国家自然科学基金国家科技部科技计划项目山东省自然科学基金山东省医药卫生科技发展计划项目山东省潍坊市科技计划项目山东省卫生和计划生育委员会科技计划资助课题

815737172013GA740103ZR2012CM0252015ws00572014WS0452014-10

2017

10.3969/j.issn.1005-1678.2017.01.003

中国生化药物杂志
南京生物化学制药研究所,全国生化制药情报中心站,中国生化制药工业协会,中国药品生物制品检定所

中国生化药物杂志

ISSN：1005-1678
年,卷(期)：2017.37(1)
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