首页|急性髓系白血病中组蛋白去甲基化酶KDM3B和JMJD1C的差异化表达

急性髓系白血病中组蛋白去甲基化酶KDM3B和JMJD1C的差异化表达

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目的 探讨白血病中组蛋白去甲基化酶KDM3B和JMJD1C受到的协同调控.方法 在多个急性髓系白血病细胞株中采用细胞遗传学分析KDM3B和JMJD1C的拷贝数情况.在急性髓系白血病细胞株NB4和HL-60中,应用Daminozide进行处理,观察组蛋白H3K9单甲基化和二甲基化的情况.在急性髓系白血病细胞株NB4和HL-60中,应用Daminozide、ATRA(All-trans retinoid acid)、Vtamin C进行处理,通过实时定量PCR检测去甲基化酶KDM3B和JMJD1C的表达情况.结果 KDM3B和JMJD1C在不同的急性髓细胞白血病细胞系中拷贝数呈负相关.经Daminozide药物分别对NB4和HL-60细胞进行处理后,NB4细胞和HL-60细胞组的H3K9单甲基化和二甲基化的水平呈上升趋势.使用3种药物分别进行处理后,在NB4细胞中,KDM3B的mRNA下调,JMJD1C的mRNA水平上调;与之相反,在HL-60细胞中,表现为KDM3B的mRNA上调和JMJD1C的mRNA水平下调.结论 急性髓细胞白血病中JMJD1C和KDM3B呈负相关的表达趋势.
Differential expression of histone demethylase KDM3B and JMJD1C in acute myeloid leukemia
Objective To investigate the synergistic regulation of KDM3B and JMJD1C in leukemia. Methods The expression level of JMJD1C and KDM3B were analyzed in multiple acute myeloid leukemia (AML) cell lines. AML cell lines NB4 and HL-60 were treated with Daminozide, followed by determination of H3K9 mono-methylation and di-methylation. AML cell lines NB4 and HL-60 were treated with Daminozide, ATRA (retinoid acid All-trans), C Vitamin and the expression of KDM3B and JMJD1C were detected by real-time quantitative PCR. Results The expression level of KDM3B and JMJD1C in the AML cell lines was negatively correlated. In NB4 and HL-60 cells treated by daminozide, H3K9 mono-methylation and di- methylation level showed a rising trend in these two cell groups. After treatment of NB4 cells with the 3 reagents, the level of mRNA of KDM3B was down-regulated while the level of mRNA of JMJD1C was up-regulated. In HL-60 cells treated by daminozide, the mRNA level of KDM3B was up-regulated and the mRNA level of JMJD1C was down-regulated. Conclusion The expression of KDM3B and JMJD1C is negatively correlated in patients with AML.

acute myeloid leukemiahistone modifying enzymesKDM3BJMJD1C

屈玟、徐鑫、赵瑶、胡振波

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潍坊医学院 研究生院,山东 潍坊 261031

潍坊医学院 临床学院,山东 潍坊 261031

潍坊医学院附属医院 干细胞与再生医学实验室,山东 潍坊 261031

急性髓系白血病 组蛋白修饰酶 KDM3B JMJD1C

国家自然科学基金国家自然科学基金教育部海外归国学者科学研究基金山东省医药卫生科技发展计划项目

8157015781370628815701572013WSB28012

2017

中国生化药物杂志
南京生物化学制药研究所,全国生化制药情报中心站,中国生化制药工业协会,中国药品生物制品检定所

中国生化药物杂志

ISSN:1005-1678
年,卷(期):2017.37(1)
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