不同刺激剂对小鼠脾脏淋巴细胞LAG3表达及功能的影响
Effect of different stimulants on the LAG3 expression and function of spleen lymphocytes in mice
薛妮娜 1王春阳 1陈越 1王东杰 1来芳芳 1陈晓光1
作者信息
- 1. 中国医学科学院药物研究所,药理室,天然药物活性物质与功能国家重点实验室/创新药物非临床药物代谢及PK/PD研究北京市重点实验室,北京 100050
- 折叠
摘要
目的 研究不同刺激剂对小鼠脾脏淋巴细胞LAG3表达及功能的影响.方法 采用密度离心法分离小鼠脾脏淋巴细胞.流式细胞术检测conA、PMA、PHA和CD3/28抗体刺激24 h或72 h对T细胞亚群LAG3表达的影响.ELISA法检测刺激剂对细胞上清IFN-γ含量的影响.MTT法检测刺激剂对淋巴细胞增殖的影响.结果 conA培养24 h或72 h可剂量依赖性增加LAG3+CD3+和LAG3+CD4+CD3+T细胞百分比.CD3/28抗体培养72 h显著增加LAG3+CD3+和LAG3+CD4+CD3+T细胞百分比.同时,conA和CD3/28抗体可时间依赖性增加淋巴细胞IFN-γ的分泌.conA、PMA、PHA和CD3/28抗体培养72 h均表现出刺激淋巴细胞增殖的作用.结论 conA和CD3/28抗体是T细胞活化的有效刺激剂,并可以促进T细胞LAG3的表达和IFN-γ的分泌.
Abstract
Objective To investigate the effect of different stimulants on the LAG3 expression and function of spleen lymphocytes in mice. Methods The spleen lymphocytes from mice were isolated by density centrifugation.The LAG3 expressions in T cell subsets after exposure to conA, PMA, PHA or anti-CD3/28 antibodies for 24 h or 72 h were analyzed by Flow cytometry.The IFN-γsecretions of conditional medium were detected by ELISA kit.The proliferation of lymphocytes was examined by MTT analysis.Results Treatment with conA for 24 h or 72 h dose-dependently increased LAG3 +CD3 +and LAG3 +CD4 +CD3 +T cell percentages.Similarly, an exposure of anti-CD3/28 antibodies for 72 h significantly increased LAG3 +CD3 +and LAG3 +CD4 +CD3 + T cell percentages.Meanwhile, conA and anti-CD3/28 antibodies increased the IFN-γsecretion of lymphocytes in a time-dependent manner.Furthermore, Treatment with conA, PMA, PHA or anti-CD3/28 antibodies for 72 h could enhance the proliferation of lymphocyte. Conclusion conA and anti-CD3/28 antibodies are effective activators of T cells, and both of them could promote the expression of LAG3 and IFN-γsecretion of lymphocytes.
关键词
淋巴细胞/淋巴细胞活化因子3/干扰素-γ/细胞增殖Key words
lymphocytes/lymphocyte activation gene 3/interferon-γ/cell proliferation引用本文复制引用
基金项目
医科院药物所创新药物发现与新技术专项(2016ZX350041)
医科院药物所创新药物发现与新技术专项(2016ZX350002)
出版年
2017
NETL
NSTL
维普
万方数据