三种方法制备盐霉素胶束的工艺比较
Comparison of the three preparation methods in technology of salinomycin micelle
高光远 1柳文洁1
作者信息
- 1. 中南大学 药学院,湖南 长沙 410013
- 折叠
摘要
目的 寻找最佳的盐霉素胶束的制备工艺.方法 以DSPE-PEG2000为载体,盐霉素纳为模型药物,分别使用薄膜分散法、乙醇注入法、透析法制备盐霉素胶束,以包封率和载药量,释放量,体外抗肿瘤细胞毒性等为综合评价指标,选出最适合盐霉素胶束的制备工艺.结果 薄膜分散法制得的盐霉素胶束平均粒径为(14±2.3)nm,包封率为(82±2.6)%,载药量为(6.3±2.1)%,对HepG2肿瘤细胞的IC50为16.10±3.71.结论 薄膜分散法所制得盐霉素胶束具有小粒径、高包封率、高载药量的优点,对肿瘤细胞有更强的杀伤性,且具有缓释作用.
Abstract
Objective To research the optimal preparation technology of salinomycin micelle.Methods DSPE-PEG2000 was selected as the carrier.Salinomycin was selected as the model drug.The film dispersion method, the ethanol injection method and the dialysis method were used to prepare salinomycin micelles respectively.The comprehensive evaluation indexes included entrapment rate and drug-loading rate, release capacity and vitro cytotoxicity test in order to select the most suitable preparation technology of salinomycin micelle .Results The film dispersion method is the most suitable preparation technology of salinomycin micelle in the three methods.Its average grain diameter was (14 ±2.3) nm, entrapment rate was (82 ± 2.6)%, drug-loading rate was (6.3 ±2.1)%, IC50 to HepG2 tumor cells was 16.10 ±3.71.Conclusion The film dispersion method of salinomycin micelles has the advantages with the smallest size, the highest entrapment rate and the largest drug-loading rate, which has the function to kill tunmor cells and release slowly.
关键词
盐霉素/胶束/薄膜分散法/包封率/释放量/体外细胞毒性Key words
salinomycin/micelle/film dispersion method/entrapment rate/release capacity/vitro cytotoxicity引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据