首页|灵芝多糖Fe(Ⅲ)配合物的合成及吸附动力学研究

灵芝多糖Fe(Ⅲ)配合物的合成及吸附动力学研究

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目的 提取灵芝多糖,确定灵芝多糖Fe(III)配合物的合成条件,并研究其吸附动力特性.方法 采用水提、醇沉、脱色、透析等方法,提取灵芝多糖,在碱性条件下,灵芝多糖水溶液与FeCl3反应,合成灵芝多糖铁配合物(GPC);采用邻菲罗啉分光光度法,分析GPC中Fe(III)在不同条件下的含量变化,确定灵芝多糖对Fe(III)的吸附动力学特性;通过红外光谱对其结构进行分析.结果 所制得的GPC为红棕色无定型粉末,易溶于水,在pH值3~12范围内不沉淀、不水解、不溶于有机溶剂;在相同温度下,灵芝多糖对Fe(III)的吸附量随时间逐渐增大,于1.5 h后基本达到稳定;在相同时间,灵芝多糖对Fe(III)的吸附量随温度的升高而增大.红外光谱检测分析证明灵芝多糖可与金属离子铁(Ⅲ)生成稳定的配合物,发生配合的基团主要是羟基,其中铁以聚合的β-FeOOH铁核结构存在于GPC中.结论 灵芝多糖作为复合体与金属铁离子结合能形成稳定的GPC,时间和温度是影响GPC制备工艺的关键条件;GPC有望开发成为一种新型理想的口服补铁剂.
Study on synthesis and adsorption kinetics of Ganoderma lucidum Polysaccharide-Fe(Ⅲ) complex
Objective To confirm the synthesis conditions of Ganoderma lucidum Polysaccharide-Fe (Ⅲ) Complex, and study on adsorption kinetics of it.Methods Adopt alcohol sedimenting technique and decoloring, dialysis, etc, extracting refined polysaccharide from Ganoderma lucidum.The solution of the Polysaccharide with FeCl3 react in alkaline conditions.To synthesize the polysaccharide iron complexes(GPC).The iron contents were determined by o-phenanthroline spectrophotography and and study on adsorption kinetics of Ganoderma lucidum Polysaccharide-Fe (Ⅲ) Complex.Results GPC is red brown and shapeless powder,it's liable to dissove in water,its solution is neutral,without precipitation,dissociation and Fe3 +at the pH from 3 to 12,and insoluble in organic solvents.So GPC is very stable.At the same time under different temperatures, the Fe3 + and polysaccharide on the adsorption quantity of increased gradually over time, the basic stability after 1.5 h.At the same time, with different temperatures the adsorption quantity of polysaccharide Fe3 + increases with the rise of temperature.Conclusion GPC may become a new ideal oral iron.Time and temperature are the key fabrication processes of GPC.

Ganoderma lucidum polysaccharidPolysaccharide-Fe( Ⅲ) complexadsorption kinetics

梁雅、张素清

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浙江省温岭市中医院 中药房,浙江 温岭 317500

灵芝多糖 多糖Fe(Ⅲ)配合物 吸附动力学

2017

中国生化药物杂志
南京生物化学制药研究所,全国生化制药情报中心站,中国生化制药工业协会,中国药品生物制品检定所

中国生化药物杂志

ISSN:1005-1678
年,卷(期):2017.37(2)
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