水蛭素提取纯化研究进展
Research progress of extraction and purification of Hirudin
黄胜 1熊伟 1姚俊杰 1李路 1陈红雕 1杨引欢 1姚洪君1
作者信息
- 1. 贵州大学 动物科学学院,贵州 贵阳 550025
- 折叠
摘要
水蛭素(Hirudin)是水蛭唾液腺分泌物中的一种蛋白,是迄今为止发现的最强的天然凝血酶特效抑制剂,具有活血、化淤和通经作用.但水蛭躯体中存在无抗凝活性的伪水蛭素组分和分泌物中大量成分复杂的物质,增加了水蛭素分离纯化难度;临床运用对生物制品的纯度要求高,世界各地很多科学家对水蛭素的提取和纯化做了许多相关的研究.因此,本综述根据相关研究,对水蛭素的提取、纯化工作进行介绍,分析各种提取及纯化方法的利弊,展望其发展趋势,以期为今后水蛭素的提取纯化提供借鉴.
Abstract
Hirudin as a novel anticoagulant and antithromotic agent, is a kind of protein that is obtained from the salivary gland secretion of one kind blood-sucking leech.It increases the difficulty extraction and purification of hirudin that composition of no anticoagulant activity of peseudo-hirudin in the body of leech and a large number of complex material from the salivary gland secretion of one kind blood-sucking leech.This article focuses on reviewing the structure of hirudin, genetic makeup, extraction and purification of hirudin according to the related research.Analysis of the pros and cons of various extraction and purification methods.Looking forward to its developing tendency, so as to provide reference for extraction and purification of Hirudin.
关键词
水蛭素/提取/纯化Key words
Hirudin/extraction/purification引用本文复制引用
基金项目
贵州大学大学生SRT项目(贵大SRT字2015)
水产养殖特色专业项目(801136110201和095号)
贵州省特色水产产业技术体系项目()
贵州省黔西南特色产业扶持与生态修复协同创新中心平台建设项目()
出版年
2017
NETL
NSTL
维普
万方数据