Study on anti-tumor and anti-angiogenic activity of cryptotanshinone
Objective To study the antitumor activity of cryptotanshinone and its mechanism of anti-angiogenic activity, providing a data for the treatment of cryptotanshinone on tumors.Methods Five kinds of tumor cells were cultured, such as colon cancer cell line HCT116, breast cancer cell line MDA-MB-231, lung cancer cell line A549, prostate cancer cell line PC-3 and clear ovarian cancer ES-2 cell.MTT assay was used to evaluate the anti-tumor effect of cryptotanshinone after co-culture with five concentrations (200, 40, 8, 1.6, 0.32 μg/mL).The cell cycle was detected by flow cytometry.Transwell assay was used to detect the effect of cell invasion.Human umbilical vein endothelial cells ( HUVEC ) were selected to further evaluate the inhibitory effect of cryptotanshinone on angiogenesis and its regulation of phosphorylated VEGFR1 and VEGFR2 proteins.Results Among the five tumor cells, cryptotanshinone had the strongest anti-tumor activity against breast cancer cell line MDA-MB-231.Cell cycle analysis showed that cryptotanshinone significantly blocked the MDA-MB-231 cells in G0/G1 phase, and cryptotanshinone significantly inhibited MDA-MB-231 cell invasion (P<0.01), the ability to inhibit the invasion was in a concentration-dependent manner at different concentrations of cryptotanshinone (P<0.01).In addition,cryptotanshinone can inhibit angiogenesis, and can significantly inhibit the phosphorylation levels of VEGFR1 and VEGFR2 in vascular endothelial cells.In particular, the inhibitory activity was more pronounced at concentrations of 10 μM and 20 μM.Conclusion Cryptotanshinone has anti-tumor and anti-angiogenic activity, is expected to become a new strategy for the treatment of cancer.
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