乌司他丁对脑缺血再灌注损伤大鼠海马神经元形态及MMP-9表达的作用研究
Studies on the effect of ulinastatin on hippocampal neuron and MMP-9 expression after ischemia-reperfusion injury rats
曹立军 1朱泽萌 2王进3
作者信息
- 1. 山东大学齐鲁医院急诊科,山东济南250012
- 2. 山东中医药大学中医学院,山东济南250355
- 3. 山东大学医学院药理学系,山东济南250012
- 折叠
摘要
目的 观察乌司他丁(UTI)干预的脑缺血再灌注损伤的大鼠海马区形态功能学及基质金属蛋白酶-9(MMP-9)表达水平变化,探讨UTI对大鼠脑缺血再灌注损伤海马区神经元的保护作用和MMP-9表达之间的关系,并分析其作用机制.方法 线栓法制备大鼠局灶性脑缺血再灌注损伤模型.采用UTT进行干预,于脑缺血再灌注24 h对大鼠海马进行尼氏染色病理学观察,采用免疫组化和图像分析技术分析缺血脑组织MMP-9的表达水平.结果 UTI可显著降低损伤脑组织中MMP-9免疫反应的校正光密度值(COD),改善保护海马神经元的结构功能.结论 UTI能保护缺血再灌注损伤大鼠海马区神经元形态,抑制MMP-9的表达.
Abstract
Objective To investigate the effect of ulinastatin on hippocampal neuron and matrix metalloproteinase-9 ( MMP-9 ) expression after ischemia-reperfusion injury rats, to reveal the mechanism of protection on hippocampus of ulinastatin.Methods The focal ischemia reperfusion model was made by thread embolism of middle cerebra1 artery, and ulinastatin was used.The animals were killed at 24 h after reperfusion was started.The sections of the brains were processed with Nissl staining and by immunohistochemistry with antibody of MMP-9.Results Corrected optical density (COD) of MMP-9 expression was significantly decreased after administration of ulinastatin.Also ulinastatin can obviously protect the structure of hippocampal neuron.Conclusion Ulinastatin could protect pyramidal cells in CA1 region from damage of ischemia-reperfusion injury and increase the number of normal neurons.Inhibition on MMP-9 expression was involved in the mechanism.
关键词
乌司他丁/再灌注损伤/海马/MMP-9Key words
Ulinastatin/reperfusion injury/hippocampus/matrix metalloproteinase-9.引用本文复制引用
基金项目
山东省医药卫生科技发展计划(2014WS0146)
出版年
2017
NETL
NSTL
维普
万方数据