首页|纤维化肾小管上皮细胞miRNA-21表达情况研究

纤维化肾小管上皮细胞miRNA-21表达情况研究

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目的 探究miRNA-21与肾纤维化之间的关系.方法 体外培养肾小管上皮细胞(NRK-52E),采用转化生长因子β1(transforming growth factorβ1,TGF-β1)制作细胞纤维化模型,采用慢病毒转染miRNA-21;加骨形态发生蛋白-7(bone morphogenetic protein-7,BMP-7)干预TGF-β1处理后的NRK-52E细胞,与阴性对照组相比,检测纤维连接蛋白1(fibronectin 1,FN1),信号转导分子1(SMAD1),SMAD6,rno-miR-21-5p的基因表达情况.结果 TGF-β1处理后,NRK-52E细胞BMP-7 mRNA及蛋白表达显著降低,FN1 mRNA及蛋白表达显著增加(P<0.05).BMP-7基因慢病毒载体转染NRK-52E细胞后,BMP-7 mRNA表达显著增加(P<0.05),FN1、SMAD6、rno-miRNA-21-5p mRNA明显下降,SMAD1表达增加(P<0.05).结论 miRNA21与TGF-β1所诱导的肾小管上皮细胞表型转化(epithelial-to-mesenchymal transition,EMT)进程呈正相关,可能与BMP-7、SMAD1及SMAD6的改变有关.
Stady on the expression of miRNA-21 in renal tubular epithelial cells
To describe the relationship between miRNA-21 and renal fibrosis.Methods NRK-52E cells cultured in vitro,then constructed fibrosis model induced by transforming growth factorβ1 ( TGF-β1 ) , then bone morphogenetic protein-7 ( BMP-7 )-containing lentivirus vector was used to transfect the NRK-52E cells to anti-fibrosis, SMAD1, SMAD6, FN1, rno-miR-21-5p gene expression were detected.Results After TGF-β1 treatment, the BMP-7 mRNA and protein expressions were significantly decreased, FN1 mRNA and protein expressions were significantly increased ( P<0.05 ) . After transfected by BMP-7-containing lentivirus vector, BMP-7 mRNA expression significantly increased(P<0.05), FN1, SMAD6 and rno-miRNA-21-5p mRNA decreased, SMAD1 mRNA increased(P<0.05).Conclusion Epithelial-to-mesenchymal transition (EMT) is positively correlated with the miRNA21 and TGF-beta 1, its maybe related to the changes of BMP-7, SMAD1 and SMAD6.

renal tubular epithelial cellsrenal fibrosismiRNA-21TGF-β1BMP-7

钟瑜、韩文伦、朱芸芸、徐再春

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浙江省立同德医院肾病科,浙江杭州310012

肾小管上皮细胞 纤维化 miRNA-21 TGF-β1 BMP-7

浙江省自然科学基金

LQ13H050002

2017

10.3969/j.issn.1005-1678.2017.03.011

中国生化药物杂志
南京生物化学制药研究所,全国生化制药情报中心站,中国生化制药工业协会,中国药品生物制品检定所

中国生化药物杂志

ISSN：1005-1678
年,卷(期)：2017.37(3)
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