Preparation of bio-electrochemical sensor based on graphene oxide nanoribbon and its application in amino acid detection
Objective To develop a bio-electrochemical based on oxidized graphene nanobelts ( GON) for rapid detection of L and D-amino acids (AA).Methods Two kinds of electrodes were prepared using two types of graphene materials, graphene oxide nanoribbons and reduced graphene nanoribbons (GRN), and modified with CSPE.H2O2, L-AA and electroactive molecular signals were detected by differential pulse voltammetry (DPV) using D-AA and D-amino acid oxidase (DAAO) reactions.In addition, two clinically relevant AA-tyrosine (Tyr) and methionine (Met), which are electrically active, were examined and the L and D enantiomers were calibrated and quantified.Finally, the content in the urine samples were detected. Results The results of this study show that L-Tyr and D-Tyr in urine samples can be accurately detected in the presence of uric acid and other electroactive AA, and the recoveries are (95 ±5)% and (99 ±3)%, respectively.Conclusion The biosensor based on GON can be used as an early diagnostic tool for D-AA related diseases, which is of great significance in clinical early diagnosis.
NETL
NSTL
万方数据
维普
