氧化石墨烯纳米带生物电化学传感器的研制及对氨基酸的检测
Preparation of bio-electrochemical sensor based on graphene oxide nanoribbon and its application in amino acid detection
张婷婷 1鄂义峰1
作者信息
- 1. 锦州医科大学药学院,辽宁锦州121000
- 折叠
摘要
目的 研制一种基于氧化石墨烯纳米带(Oxidized graphene nanobelts,GON)修饰的生物电化学传感器,用于L和D-氨基酸(AA)的快速检测.方法 采用2种石墨烯材料,氧化石墨烯纳米带(GON)和还原石墨烯纳米带(reduced graphene nanoribbons,GRN),修饰于丝网印刷碳电极(CSPE)制备2种电极.利用D-AA和D-氨基酸氧化酶(DAAO)反应产生H2O2,然后以制备的电极通过差分脉冲伏安法(DPV)检测H2O2和L-AA电信号,并对电极和酶反应条件进行优化.选择具有电活性的酪氨酸(Tyr)为目标分析物,并对L-Tyr和D-Tyr进行定量分析,最后对其在尿样品中的Tyr含量进行检测.结果 在尿酸和其他电活性AA的存在下,该生物电化学传感器能够精确的检测出尿样中的L-Tyr和D-Tyr含量,其回收率分别为(95±5)%和(99±3)%.结论 该基于GON的电化学生物传感器可作为D-AA相关疾病的早期诊断工具,在临床早期诊断具有重要意义.
Abstract
Objective To develop a bio-electrochemical based on oxidized graphene nanobelts ( GON) for rapid detection of L and D-amino acids (AA).Methods Two kinds of electrodes were prepared using two types of graphene materials, graphene oxide nanoribbons and reduced graphene nanoribbons (GRN), and modified with CSPE.H2O2, L-AA and electroactive molecular signals were detected by differential pulse voltammetry (DPV) using D-AA and D-amino acid oxidase (DAAO) reactions.In addition, two clinically relevant AA-tyrosine (Tyr) and methionine (Met), which are electrically active, were examined and the L and D enantiomers were calibrated and quantified.Finally, the content in the urine samples were detected. Results The results of this study show that L-Tyr and D-Tyr in urine samples can be accurately detected in the presence of uric acid and other electroactive AA, and the recoveries are (95 ±5)% and (99 ±3)%, respectively.Conclusion The biosensor based on GON can be used as an early diagnostic tool for D-AA related diseases, which is of great significance in clinical early diagnosis.
关键词
氧化石墨烯纳米带/氨基酸/生物电化学传感器Key words
oxidized graphene nanobelts/amino acids/bio-electrochemical引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据