功能化高分子脂质体包裹的纳米金颗粒作为新型CT造影剂的初步研究
Preliminary research on functional polymer liposome-entrapped gold nanoparticles as CT contrast agents
郭妍妍 1刘雅娟 2王琳 1李欢 1常津 2李小东1
作者信息
- 1. 天津大学 生命科学学院,天津 300072
- 2. 天津医科大学第二医院 放疗科,天津 300211
- 折叠
摘要
目的 探讨功能化高分子脂质体包裹的纳米金(LADL@AuNPs)作为分子探针用于CT造影的可行性.方法 采用超声微乳液法将纳米金颗粒包裹到自主研发的亚油酸改性葡聚糖高分子脂质体中,以相同浓度临床成像剂碘海醇作对照,进行体外CT扫描,分析2者的CT值差异.采用合适浓度(6mg/mL)颗粒悬液,尾静脉注射到Balb/c裸鼠体内,实时观察CT显影.结果 体内外实验表明大于4mg/mL颗粒的CT值远高于相同条件下碘海醇,也可更清晰的显示器官对比,且具有更长的体内循环时间.结论 相比于碘海醇,功能化高分子脂质体包裹的纳米金颗粒可用作新型CT分子探针,具有极大应用前景.
Abstract
Objective To research the functional polymer liposome-trapped gold nanoparticles as molecular probes used as CT contrast agents.Methods Gold nanoparticles were entrapped by ultrasonic micro-emulsion method using self-developed linoleic acid-modified dextran polymer liposome.Under the same concentrations,clinical imaging was performed with Iohexol as control for the analysis of CT value differences.Then,the particle suspensions at appropriate concentration(6 mg/mL)were intravenously injected into Balb/c nude mice for real-time observation of CT imaging.ResultsIn vitro results showed that nanoparticles at greater than 4 mg/mL could present much higher CT value than Iohexol at the same conditions,while in vivo studies indicated nanoparticles could clearly show different organ contrast with prolonged circulation time.Conclusion Compared to clinical contrast agent Iohexol,functional polymer liposome entrapped gold nanoparticles could possess a great application prospect to be used as a new type of molecular probes for CT enhanced imaging.
关键词
纳米金/高分子脂质体/CT成像/造影剂/分子探针Key words
gold nanoparticles/polymer liposome/CT imaging/contrast agent/molecular probes引用本文复制引用
基金项目
天津市自然科学基金重点项目(16JCZDJC35100)
国家自然科学基金(51373117)
天津市卫生行业重点科研攻关基金(15KG137)
出版年
2017
NETL
NSTL
维普
万方数据