虎眼万年青提取物对白色念珠菌细胞凋亡的影响
Effect of ethanolic extract of Omithogalum caudatum Ait on apoptosis of Candida albicans
王楠 1孟美竹 1谢鲲鹏 1谢明杰1
作者信息
- 1. 辽宁师范大学生命科学学院,辽宁省生物技术与分子药物研发重点实验室,辽宁 大连 116081
- 折叠
摘要
目的 研究虎眼万年青(OCA)提取物对白色念珠菌细胞凋亡的影响,阐述虎眼万年青抑真菌的可能作用机制.方法 采用AnnexinⅤ-FITC/PI双染检测OCA对白色念珠菌凋亡的影响;JC-1染色检测OCA对线粒体膜电位的影响;DCFH-DA染色检测OCA对活性氧生成的影响.结果 OCA提取物对白色念珠菌有较强的抑制作用,其对白色念珠菌的MIC80与MFC分别为8 mg/mL与32 mg/mL.虎眼万年青提取物能诱导细胞凋亡,减低白色念珠菌的线粒体膜电位,显著增加白色念珠菌的活性氧含量.结论 虎眼万年青提取物抑制真菌的作用机制可能与降低白色念珠菌的线粒体膜电位,提高活性氧的含量,扰乱线粒体功能,最终导致其细胞凋亡有关.
Abstract
Objective To investigate the effect of Omithogalum caudatum Ait(OCA)on apoptosis of Candida albicans,illustrated the antifungal mechanism of OCA.Methods Annexin Ⅴ-FITC/PI double stainingwas used to detect the effect of OCA on the apoptosis of C.albicans;JC-1 and DCFH-DA staining were used to detectthe effect of OCA on mitochondrial membrane potential(MTP)and reactive oxygen species(ROS)of C.albicans.Results OCA had a good antifungal activity,the minimum inhibitory concentration(MIC)and the minimum fungicidal concentration(MFC)were 8mg/mL and 32mg/mL respectively.OCA could induceapoptosis of C.albicans,promote the reduction of MTP and increase of ROS.Conclusion OCA induced cell apoptosismainly through disrupting mitochondrial function.
关键词
虎眼万年青/白色念珠菌/细胞凋亡Key words
Omithogalum caudatum Ait/Candida albicans/cell apoptosis引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据