非小细胞肺癌中EGFR及多药耐药蛋白与化疗疗效的关系
Relationship between EGFR and multidrug resistance protein in non-small cell lung cancer
徐铖 1罗华荣 1甘梅富1
作者信息
- 1. 台州恩泽医疗中心(集团)病理学部,台州恩泽医院病理科,浙江台州318000
- 折叠
摘要
目的 探讨表皮生长因子受体(EGFR)与多药耐药蛋白的表达及与化疗疗效的关系.方法 采用免疫组化二步法检测95例非小细胞肺癌患者肿瘤组织和15例正常肺组织中EGFR与多药耐药蛋的表达.结果 EGFR表达在有无吸烟史、不同病理类型(鳞癌vs.腺癌)、不同TNM分期及有无淋巴结转移的NSCLC患者之间有统计学差异(P<0.05).LRP和MRP在腺癌中的阳性表达率均高于鳞癌(P<0.05),MRP表达在高分化癌的阳性率高于中-低分化(P<0.05).MRP与LRP、PGP与GST-π的表达呈正相关性(r=0.341,P=0.001;r=0.213,P=0.038),EGFR与LRP、P-gp、MRP、GST-π的表达均无明显相关性.EGFR蛋白过度表达的抗肿瘤治疗有效率37.5%低于EGFR阴性的有效率62.5%(P<0.05).结论 NSCLC组织中EGFR与多药耐药蛋白常过度表达.形成MDR的四种蛋白之间相互联系,可能对耐药的形成起协同作用,EGFR可以作为评价NSCLC化疗疗效和疗效的客观指标.
Abstract
Objective To investigate the expression of epidermal growth factor receptor (EGFR) and multidrug resistance protein, and it's relationship with efficacy of chemotherapy in NSCLC.MethodsThe immunohistochemical (EnVision method) was used to detect the expression of EGFR and LRP, P-gp, MRP, GST-π in 95 cases of NSCLC tissues and 15 cases of normal lung samples.ResultsEGFR in NSCLC tissues was significantly correlated with the history of smoking, pathological types of cancer(squamous cell carcinoma vs adenocarcinoma), presence of lymph node metastasis, and TNM stages (P< 0.05).The positive expression rate of LRP and MRP in adenocarcinoma were higher than squamous cell Carcinoma (P< 0.05), and MRP in high differentiate cancer was higher than low differentiate cancer (P< 0.05).The expression of MRP and LRP, PGP and GST-π in NSCLC were positive correlation(r=0.341, P=0.001;r=0.213, P=0.038), there is no correlation between EGFR with LRP, P-gp, MRP, GST-π.EGFR-negative chemotherapy efficiency (62.5%) was significantly higher than EGFR-positive(37.5%, P< 0.05).ConclusionEGFR and multi drug resistance proteins are overexressed in NSCLC tissues.multi drug resistance proteins have a synergistic to drug resistance.EGFR could be used to objectively predict chemotherapy effect and prognosis of NSCLC.
关键词
非小细胞肺癌/免疫组织化学/表皮生长因子受体/多药耐药蛋白Key words
non-small cell lung cancer/immunohistochemical/epidermal growth factor receptor/multi-drug resistance proteins引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据