摘要
目的 分析血液标本放置时间与临床生化检验结果相关性,为临床检验提供参考.方法 选取杭州市富阳区第二人民医院需要生化检验的待测血液标本100份作为研究对象,采用全自动生化仪结合电解质分析仪检测不同存放时间(1h、3h、6h、24h)下生化检验指标数据.结果 以采血1h测定的结果作为检验标准,标本放置3h的结果显示,葡萄糖含量、CO2含量测定值均显著下降(P<0.05);标本放置8h,血钾、葡萄糖、CO2、谷丙转氨酶测定值存在显著差异(P<0.05);标本放置24h,血钾、葡萄糖、CO2、谷丙转氨酶、碱性磷酸酶、总胆红素、总蛋白、白蛋白、谷草转氨酸、血钾、α-羟丁酸脱氢酶、乳酸脱氢酶与放置1h相比,差异具有统计学意义(P<0.05).结论 血液标本放置时间会对血糖、CO2等生化指标有显著影响,针对血糖、CO2指标应在血液标本1h内完成检测,避免耽误病情评估.
Abstract
Objective To analyze relevant mechanism of clinical biochemical test results with blood specimen placement time,and provide reference for clinical examination.Methods100 biochemical test specimen in our hospital were selected, Biochemical test data were analyzed at different storage time (1h, 3h, 6h, 24h) using automatic biochemical analyzer combined with electrolyte analyzer.ResultsThe results of blood 1H determination were taken as the test standard, and the blood samples were placed in 3h.The results showed that the contents of glucose and carbon dioxide were significantly decreased (P<0.05).Blood samples were placed 8h, serum potassium, glucose, carbon dioxide, alanine aminotransferase (P<0.05) were significant differences, blood specimens were placed 24h, there were significant differences of the indexes including blood glucose, potassium, carbon dioxide, alanine aminotransferase, alkaline phosphatase, total bilirubin, total protein, albumin, aspartate aminotransferase, serum potassium, alpha hydroxybutyrate dehydrogenase, lactate dehydrogenase (P<0.05).ConclusionThe blood sample placement time have a significant impact on some biochemical indicators, in view of the unstable factors should be detected in the blood sample 1h intact, to avoid delay condition assessment.