Study on the cholera toxin B subunit displaying HIV-1 gp41 membrane-proximal external region epitope
Objective To evaluate the immune effect of fusion protein of CTB and HIV-1 gp41 MPER epitope.MethodsThe cholera toxin B subunit was used as a carrier to display the epitopes of bNAbs 2F5 and 4E10 targeting the MPER.The purified fusion proteins with Freund adjuvant were immunized in mice and the immunized sera were analyzed after the four times immunization.The immunogenicity of the fusion proteins was analyzed by ELISA.And the IgA antibodies were detected to evaluate the mucosal immune response in mice.The different types of antibodies and the cytokines INF-γ and IL-4 of PBMC were used to evaluate the immune response pathway.Besides, neutralization assay was carried out to detected the antiviral ability of immunized serum.ResultsCTB as a carrier has good immunogenicity and fusion proteins can induced specific antibodies against MPER epitopes.In addition, the fusion proteins stimulated mucosal immunity, induced IgA, IgG1, IgG2a and IgG2b antibodies and the Th2 immune response pathway in mice.Importantly, CTB-4E10 immune serum had the neutralizing activity against the NL4-3 virus.Conclusion CTB fusion proteins had good immunogenicity and could induce humoral immunity, mucosal immunity and Th2 immune response pathway in mice.The serum from the CTB-4E10 immunized mice could inhibit virus infecting cells.This study laid the foundation for the exploration of HIV-1 gp41 MPER as candidate vaccine.
HIV-1 gp41 MPERcholera toxin B subunitimmune response
NETL
NSTL
万方数据
维普
