摘要
目的 本实验主要探究LPS诱导卵巢癌细胞上清白介素-6(IL-6)的能力,IL-6对卵巢癌细胞中EZH2基因mRNA和蛋白表达的影响.方法 培养卵巢癌细胞,根据培养基成分不同,建立试验组与对照组,采用ELISA方法检测卵巢癌细胞上清液中IL-6的含量,MTT法检测各组的OD值;Western Blotting方法检测EZH2蛋白表达;RT-PCR检测EZH2的mRNA表达.结果 试验组与对照组相比:卵巢癌细胞上清液中IL-6显著升高,为差异有统计学意义(P<0.05),卵巢癌细胞的增殖受到抑制,为差异有统计学意义(P<0.05);EZH2蛋白表达量显著降低,为差异有统计学意义(P<0.05);EZH2基因mRNA含量显著降低,为差异有统计学意义(P<0.05).结论 LPS可诱导卵巢癌细胞表达IL-6,抑制细胞增殖;IL-6具有抑制EZH2表达的作用.
Abstract
Objective The aim of this study was to investigate the expression of IL-6 induced by LPS in ovarian cancer and the effect of IL-6 on the expression of EZH2 mRNA and protein in ovarian cancer cell lines.MethodsCulture ovarian cancer cell lines, according to the different medium composition, establish the experimental group and the control group, detect the concentration of IL-6 in the supernatant of ovarian cancer cells by ELISA and the OD values of each group by MTT assay.Western blotting was used to detect the expression of EZH2 protein in ovarian cancer cells.The mRNA expression of EZH2 was detected by RT-PCR.ResultsThe expression of IL-6 in the supernatant of ovarian cancer cells was significantly higher than that in the control group (P<0.05).The proliferation of ovarian cancer cells was inhibited and the difference was statistically significant (P<0.05).The expression of EZH2 protein in ovarian cancer cells was significantly decreased, and there was statistical difference (P<0.05).The mRNA expression of EZH2 was significantly decreased, and there was statistical difference (P<0.05).ConclusionLPS could induce the expression of IL-6 and inhibit cell proliferation in ovarian cancer cells.IL-6 could inhibit the expression of EZH2.
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维普
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