酶联免疫法检测乙肝PreS1抗原及抗体在乙肝诊断中的应用价值
The application value of enzyme linked immunosorbent assay for hepatitis B PreS1 antigen and antibody in the diagnosis of hepatitis B
高丽 1胡焉凡1
作者信息
- 1. 金华市中医医院 检验科,浙江 金华 321000
- 折叠
摘要
目的 探究酶联免疫法检测乙肝PreS1抗原及抗体在乙肝诊断中的应用价值.方法 选择2014年8月~2016年8月乙肝"两对半"检查结果中几种常见模式为样本,采用乙肝"两对半"7种常用检测模式对140例行PreS1抗原、抗体检测.经酶联免疫法检测PreS1抗原及抗体.结果 在大三阳患者中PreS1抗原阳性87.5%,在小三阳患者中PreS1抗原阳性8.6%,在HBsAg、HBcAb阳性患者中PreS1抗原阳性13.3%,在单纯HBsAg阳性患者中PreS1抗原阳性4.3%.HBV-DNA总阳性率与PreS1总阳性率对比差异无统计学意义,且两者总符合率较高.PreS1抗原阳性与HBsAg、HBeAg及HBcAb阳性相关(P<0.05);与HBsAb、HBeAb阳性无关.结论 经乙肝PreS1抗原及抗体检测,可有效补充乙肝诊断,及早发现已存在的乙肝感染.
Abstract
Objective To explore the application value of enzyme linked immunosorbent assay for detection of hepatitis B PreS1 antigen and antibody in the diagnosis of hepatitis B.MethodsSeveral common patterns in the results of hepatitis B"two half"check were selected from August 2014 to August 2016 for the sample, the use of hepatitis B"two half"7 commonly used detection model for 140 cases of PreS1 antigen, antibody detection.Enzyme linked immunosorbent assay for detection of PreS1 antigen and antibody.ResultsIn patients with HBeAg in PreS1 antigen positive rate was 87.5%, in patients with positive PreS1 antigen positive rate was 8.6%, HBsAg, HBcAb in patients with positive PreS1 antigen positive 13.3%, in a pure HBsAg in patients with positive PreS1 antigen 4.3%.There was no significant difference in the total positive rate of HBV-DNA and the total positive rate of PreS1, and the total coincidence rate was higher.PreS1 antigen positive correlated with HBsAg, HBeAg and HBcAb positive(P<0.05), and has nothing to do with HBsAb, HBeAb positive.ConclusionHepatitis B PreS1 antigen and antibody detection, can effectively complement the diagnosis of hepatitis B, early detection of hepatitis B infection.
关键词
酶联免疫法/乙肝PreS1抗原/抗体/诊断Key words
enzyme linked immunosorbent assay/hepatitis B PreS1 antigen/antibody/diagnosis引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据