大孔树脂吸附法富集山楂叶总黄酮的工艺研究
Study on Enrichment Process of Total Flavonoids from Crataegus pinnatifida Bge, with Macroporous Resin
赵玉华 1岑明政 2范海珠2
作者信息
- 1. 浙江中医药大学,浙江 杭州 310053;浙江萧山医院 药剂科,浙江 杭州 311202
- 2. 浙江省中医院,浙江 杭州310006
- 折叠
摘要
目的 研究大孔树脂富集山楂叶总黄酮的工艺条件和参数.方法 以山楂叶总黄酮含量为考察指标,采用正交试验设计,选用L9(34)正交试验表,考察上样量、径高比、洗脱溶剂用量和吸附时间4个因素对富集效果的影响.结果 最佳工艺条件为:树脂量:上样量为2:1,洗脱溶剂用量/mL:上样量为4:1,径高比为1:6,静置吸附时间为1 h.结论 采用此法可较好地富集山楂叶中的总黄酮成分.
Abstract
Objective To study the optimal technology parameters of the enrichment process for total flavonoids of Crataegus pinnatifida Bge,with macroporous Resin. Methods The enrichment process was studied by orthogonal experiment design using orthogonal from L9(34) with the content of total flavonoids from Crataegus pinnatifida Bge,as indexes, the factors such as adsorption capacity, the column diameter-height ratio, eluant volume and adsorption time were investigated. Results The optimum elution parameters were as follows: the eluant volume of 60% alcohol—the wet resin amount (g)—the sample volume (mL) was 2:1, the sample was 4:1, the column diameter-height ratio was 1:6 , the adsorption time was 1h, respectively. Conclusion This process enriching the total flavonoids of Crataegus pinnatifida Bge,is feasible.
关键词
山楂叶/总黄酮/大孔树脂/富集/正交试验Key words
Crataegus pinnatifida Bge/total flavonoids/macroporous resin/enrichment/orthogonal experiment引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据