基于盲肠结扎穿孔脓毒症大鼠动物模型的肺部炎症及氧化应激观察
Based on the cecum ligation perforation animal models of sepsis in the rat lung inflammation and oxidative stress observation
蔡莉娟 1张艳 1丁学军 1陈宗伟1
作者信息
- 1. 浙江中医药大学附属第三医院 重症医学科,浙江 杭州 310005
- 折叠
摘要
目的 基于盲肠结扎穿孔脓毒症模型,采用大鼠动物作为实验对象,观察其肺部炎症以及氧化应激.方法 将成长期在8周的雄性大鼠随机分组为对照组、假手术组和模型组3组,采用盲肠结扎穿孔术(CLP)制备脓毒症ALI模型,为达到实验观察效果,在手术12 h药物处死实验大鼠,进行肺组织HE染色分析,并观察MPO及MDA水平.结果 模型组大鼠肺组织损伤程度、MPO及MDA水平较对照组及假手术组显著增高(P<0.01).结论 基于盲肠结扎穿孔脓毒症大鼠动物模型可以很好的模拟脓毒症炎症反应及氧化应激.
Abstract
Objective To observe the pulmonary inflammation and oxidative stress in rats with cecal ligation and puncture sepsis. Methods The male rats in 8 weeks were randomly divided into control group, sham operation group and model group,The ALI model of sepsis was prepared by cecal ligation and puncture (CLP). In order to achieve the effect of experimental observation, the rats were killed after the operation 12 h, and the lung tissue HE staining analysis was performed, and the levels of MPO and MDA were observed. Results The degree of lung injury and the levels of MPO and MDA in the model group were significantly higher than those in the control group and the sham operation group (P < 0.01).Conclusion Rats with cecal ligation and puncture sepsis model can well simulate sepsis inflammatory response and oxidative stress.
关键词
脓毒症/大鼠动物/氧化应激Key words
sepsis/rat animals/oxidative stress引用本文复制引用
基金项目
浙江省中医药科技项目(2015ZB073)
出版年
2017
NETL
NSTL
维普
万方数据