目的 探索整合素-金属蛋白酶33(A Disintegrin And Metalloproteinase 33,ADAM33)基因T2位点多态性与支气管哮喘的相关性.方法 研究对象选取本院呼吸内科2015年6月～2016年12月期间收治的哮喘患儿120例作为观察组,同期入本院体检的120名健康儿童作为对照组.患儿入院均行上肢肘静脉抽血并提取DNA,随后进行聚合酶链反应-限制性片段长度多态性分析(Polymerase Chain Reaction-Restriction Fragment Length Polymorphism,PCR-RFLP)和测序分型.比较2组患儿按照Hardy-Weinberg平衡定律检验结果 、T2位点基因型分布频率以及T2位点基因频率分布及其与哮喘相对危险度.结果 2组受试对象T2位点基因频率均满足Hardy-Weinberg平衡定律,具有良好的群体代表性;观察组受试对象T2等位基因中A的基因频率明显高于对照组,2组受试对象比较差异有统计学意义(χ2=8.09,P=0.00),且A等位基因的增加是患哮喘的高危因素(OR=2.32);观察组受试对象T2位点AG基因型频率明显高于对照组,2组受试对象间比较差异有统计学意义(χ2=4.21,P=0.04),且AG等位基因的增加是患哮喘的高危因素(OR=1.89).结论 ADAM33基因T2位点多态性与支气管哮喘明显相关,其能显著增加患支气管哮喘的可能性.
Relationship between T2 locus polymorphism of ADAM33 gene and bronchial asthma
Objective To explore the relationship between the polymorphism of integrin-metalloproteinase-33 (ADAM33) gene and bronchial asthma. Methods Subjects were selected from the Department of Respiratory Medicine in our department from June 2015 to December 2016 in the treatment of 120 cases of asthma patients as the observation group, the same period into our hospital physical examination of 120 healthy children as the control group. The patients were enrolled in the upper extremity elbow vein and extracted DNA, followed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing. The frequency of T2 genotype distribution and the frequency distribution of T2 locus and the relative risk of asthma were compared according to Hardy-Weinberg equilibrium law. Results The frequency of A gene in the T2 allele of the subject was significantly higher than that of the control group. The frequency of A gene in the T2 allele was significantly higher than that in the control group (P<0.05) There was significant difference between the two groups (χ2=8.09, P=0.00), and the increase of A allele was the risk factor of asthma (OR=2.32); the observation group T2 gene AG (χ2=4.21, P=0.04), and the increase of AG allele was the risk factor of asthma. The frequency of asthma was significantly higher than that of control group (OR=1.89). Conclusion ADAM33 gene T2 locus polymorphism is significantly associated with bronchial asthma, which can significantly increase the risk of bronchial asthma.
bronchial asthmaa disintegrin and metalloproteinase 33allelegene polymorphism
NETL
NSTL
万方数据
维普
