首页|酶联免疫吸附试验检测丙型肝炎抗体的可比性研究

酶联免疫吸附试验检测丙型肝炎抗体的可比性研究

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目的 探讨酶联免疫吸附试验(Enzyme Linked Immunosorbent Assay,ELISA)检测丙型肝炎抗体的临床应用效果.方法 200例丙型肝炎患者均分别接受酶联免疫吸附试验、金标法检测丙型肝炎抗体,记录其检测结果 ,将所得数据输入SPSS软件后给予相应分析并得出结论 .结果 200例丙型肝炎患者均顺利完成金标法、ELISA法检测丙型肝炎抗体,经分析可知ELISA法检测丙型肝炎抗体阳性率显著高于金标法,其中ELISA法阳性率为82.50%、金标法阳性率为50.00%,提示ELISA法对丙型肝炎的检出率更优,数据对比差异具有统计学意义(P<0.05).结论 应用ELISA法可获得较为理想的丙型肝炎抗体检测结果 ,对提高丙型肝炎诊断正确率具有积极意义,有利于保障患者疗效、生活质量.
Comparative study of ELISA in the detection of antibodies to hepatitis C virus
Objective To investigate the clinical effect of enzyme linked immunosorbent assay (ELISA) in detecting hepatitis C antibody. Methods 200 cases of patients with hepatitis C was measured by ELISA, the gold standard method for detection of hepatitis C virus antibody, recording the test results, the data input SPSS software to give the corresponding analysis and conclusions. Results 200 cases of patients with hepatitis C were successfully completed the gold standard method and ELISA method for detection of hepatitis C virus antibody, the analysis showed that positive rate of ELISA method for detection of hepatitis C virus antibody was significantly higher than that of the gold standard method, the positive rate of ELISA was 82.50%, the positive rate of the gold standard method for was 50.00%, results suggested that HCV detection rate of ELISA method is significantly better (P<0.05). Conclusion The method of ELISA can be used for the detection of hepatitis C antibody, which has positive significance in improving the diagnostic accuracy of hepatitis C, and is beneficial to protect the efficacy and quality of life of patients.

hepatitis C antibodiesenzyme-linked immunosorbent assaycolloidal gold methodapplication effect

余燕儿、庄晓玲

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舟山市第二人民医院 检验科,浙江 舟山 316000

浙江省舟山医院 检验中心,浙江 舟山 316000

丙型肝炎抗体 酶联免疫吸附试验 胶体金法 应用效果

2017

中国生化药物杂志
南京生物化学制药研究所,全国生化制药情报中心站,中国生化制药工业协会,中国药品生物制品检定所

中国生化药物杂志

ISSN:1005-1678
年,卷(期):2017.37(9)
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