酶联免疫吸附试验检测丙型肝炎抗体的可比性研究
Comparative study of ELISA in the detection of antibodies to hepatitis C virus
余燕儿 1庄晓玲2
作者信息
- 1. 舟山市第二人民医院 检验科,浙江 舟山 316000
- 2. 浙江省舟山医院 检验中心,浙江 舟山 316000
- 折叠
摘要
目的 探讨酶联免疫吸附试验(Enzyme Linked Immunosorbent Assay,ELISA)检测丙型肝炎抗体的临床应用效果.方法 200例丙型肝炎患者均分别接受酶联免疫吸附试验、金标法检测丙型肝炎抗体,记录其检测结果 ,将所得数据输入SPSS软件后给予相应分析并得出结论 .结果 200例丙型肝炎患者均顺利完成金标法、ELISA法检测丙型肝炎抗体,经分析可知ELISA法检测丙型肝炎抗体阳性率显著高于金标法,其中ELISA法阳性率为82.50％、金标法阳性率为50.00％,提示ELISA法对丙型肝炎的检出率更优,数据对比差异具有统计学意义(P<0.05).结论 应用ELISA法可获得较为理想的丙型肝炎抗体检测结果 ,对提高丙型肝炎诊断正确率具有积极意义,有利于保障患者疗效、生活质量.
Abstract
Objective To investigate the clinical effect of enzyme linked immunosorbent assay (ELISA) in detecting hepatitis C antibody. Methods 200 cases of patients with hepatitis C was measured by ELISA, the gold standard method for detection of hepatitis C virus antibody, recording the test results, the data input SPSS software to give the corresponding analysis and conclusions. Results 200 cases of patients with hepatitis C were successfully completed the gold standard method and ELISA method for detection of hepatitis C virus antibody, the analysis showed that positive rate of ELISA method for detection of hepatitis C virus antibody was significantly higher than that of the gold standard method, the positive rate of ELISA was 82.50％, the positive rate of the gold standard method for was 50.00％, results suggested that HCV detection rate of ELISA method is significantly better (P<0.05). Conclusion The method of ELISA can be used for the detection of hepatitis C antibody, which has positive significance in improving the diagnostic accuracy of hepatitis C, and is beneficial to protect the efficacy and quality of life of patients.
关键词
丙型肝炎抗体/酶联免疫吸附试验/胶体金法/应用效果Key words
hepatitis C antibodies/enzyme-linked immunosorbent assay/colloidal gold method/application effect引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据