摘要
目的 探究车前草总黄酮对肾脏草酸钙结石大鼠模型的作用及机制.方法 50只大鼠分为对照组、模型组、车前草总黄酮高剂量组、车前草总黄酮低剂量组、阳性药物组,复制肾脏草酸钙结石大鼠模型.车前草总黄酮组大鼠给予车前草总黄酮干预,阳性药物组给予葛根素干预,连续28天.检查各组大鼠肾脏的病理、尿液中的草酸和Ca2水平、血清中的超氧化物歧化酶(SOD)和丙二醛(MDA)、肾脏中的骨桥蛋白.结果 车前草总黄酮高剂量组、车前草总黄酮低剂量组和阳性药物组大鼠的肾小管有扩张现象以及肾小管上皮细胞有坏死的现象,但程度均较模型组显著改善,在肾小管处可见无色透明的草酸钙结晶,但结晶块大小及数量均显著小于模型组;车前草总黄酮高剂量组、车前草总黄酮低剂量组、阳性药物组大鼠尿液中的草酸和Ca2水平、血清中MDA水平、肾脏中骨桥蛋白表达水平均显著低于模型组(P<0.05),血清中SOD水平显著高于模型组(P<0.05);车前草总黄酮高剂量组尿液中的草酸和Ca2水平、血清中MDA水平、肾脏中骨桥蛋白表达水平均显著低于车前草总黄酮低剂量组(P<0.05),血清中SOD水平显著高于低剂量组(P<0.05),且上述指标与阳性药物组差异无统计学意义.结论 车前草总黄酮对肾脏草酸钙结石的形成有抑制作用,其作用机制可能为抗氧化应激和抑制骨桥蛋白的表达.
Abstract
Objective To explore the effect and mechanism of total flavones of plantago asiatica for rats with calcium oxalate stone in kidney. Methods 50 rats were divided into control group, model group, total flavones of Plantago asiatica high dose group, total flavones of Plantago asiatica low dose group and positive drug group. Made the model rats with calcium oxalate stone in kidney. Rats in total flavones of plantago asiatica group were intervened by total flavonoids of plantago asiatica, the positive drug group was given puerarin intervention and continuous intervention for 28 days. The pathology of the kidneys, the levels of oxalate and Ca2 in urine, the superoxide dismutase (SOD) and malondialdehyde (MDA) in serum, and osteopontin in the kidneys were examined. Results There were dilated renal tubules and necrosis of renal tubular epithelial cells in total flavones of plantago asiatica group, but the extent of renal tubular cells was significantly improved. The urine oxalate and Ca2 levels, serum MDA levels, and osteopontin levels in the kidney in the total flavonoids of Plantago high dose group, the total flavonoids of Plantago low dose group, positive drug group were significantly lower than that in model group (P<0.05), and serum SOD level was significantly higher than that in model group (P<0.05) . The urine oxalate and Ca2 levels, serum MDA levels, and osteopontin levels in the kidney in the total flavonoids of Plantago high dose group were significantly lower than that in the total flavonoids of Plantago low dose group (P<0.05), and serum SOD level was significantly higher than that in the total flavonoids of Plantago low dose group (P<0.05) . There was no significant difference between the total flavonoids of Plantago high dose group and the positive drug group for above indexes. Conclusions The total flavonoids of pantago asiatica can inhibit the formation of renal calcium oxalate stone, and its mechanism may be the antioxidant stress and the inhibition of osteopontin expression.
基金项目
天津卫生局中医 中西医结合基金(2013JYL015)
NETL
NSTL
维普
万方数据