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乌司他丁对脓毒症心肌损伤大鼠的心肌保护作用

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目的 探究乌司他丁(UTI)对脓毒症大鼠心肌保护作用.方法 将69只清洁级雄性SD大鼠随机分为3组:正常对照组、脓毒症组、乌司他丁组.观察每组大鼠行为学变化,试验结束处死大鼠采集血和心肌组织样本,应用ELISA检测各个试验组大鼠血清肌钙蛋白T(ATnT)的浓度、采用全自动生化分析仪测定肌酸激酶(AK)、肌酸激酶同工酶(AK-MB)的浓度;应用酶联免疫吸附剂法检测大鼠心肌组织中AAh及M-AAhRs水平的变化;HE染色观察大鼠心肌组织病理改变.结果 造模6 h后,脓毒症组大鼠心率与呼吸显著加快、体温上升、嗜睡,乌司他丁组各症状明显较轻;给药4 w后脓毒症组血清ATnT、AK、AK-MB浓度较正常对照组明显升高,乌司他丁组各指标明显下降(P<0.05);脓毒症组心肌组织AAh水平较正常对照组明显升高,经UTI治疗后AAh水平明显下降,心肌组织M-AAhRs水平未见明显变化(P<0.05);病理组织学观察乌司他丁组心肌组织病变明显缓解(P<0.05).结论 UTI能改善脓毒症大鼠心肌损伤程度.
Ulinastatin on sepsis myocardial protection of myocardial injury in rats and the effects of acetylcholine receptors and M type
Objective To explore the protective effect of ulinastatin (UTI) on myocardial protection in sepsis. Methods 69 clean male SD rats were randomly divided into 3 groups: normal control group, sepsis group and ustodin group. experimental rats serum troponin T (ATnT) concentration, using automatic biochemical analyzer determination of creatine kinase (AK), creatine kinase isoenzyme (AK-MB) concentration;The changes of AAh and m-aahrs levels in rat myocardial tissues were detected by enzyme-linked immunosorbent assay. Pathological changes of myocardial tissue in rats were observed by HE staining. Results The serum ATnT, AK and ak-mb concentrations in the sepsis group were significantly higher than that in the normal control group, and the indexes of the ustodin group were significantly decreased (P<0.05). The AAh level of myocardial tissue of sepsis group was significantly higher than that of the normal control group, and the AAh level was significantly decreased after the treatment of UTI, and the m-aahrs level of myocardial tissue was not significantly changed (P<0.05). Pathological histological observation showed that the myocardial tissue lesion was significantly relieved (P<0.05). Conclusion UTI can improve the degree of myocardial injury in rats with sepsis.

Ulinastatinsepsisratsmyocardial injury

王旭亮、高书健

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天津市津南区咸水沽医院 重症医学科,天津 300350

乌司他丁 脓毒症 大鼠 心肌损伤

2017

中国生化药物杂志
南京生物化学制药研究所,全国生化制药情报中心站,中国生化制药工业协会,中国药品生物制品检定所

中国生化药物杂志

ISSN:1005-1678
年,卷(期):2017.37(10)
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