首页|竹荪和灵芝灰分对肿瘤细胞的抑制作用

竹荪和灵芝灰分对肿瘤细胞的抑制作用

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目的 研究竹荪和灵芝灰分对肿瘤细胞的抑制作用.方法 采用MTT法分别测定竹荪和灵芝醇提物及其与竹荪和灵芝醇灰分联用后对MCF-7,Hela和A375细胞的抑制作用.结果 MTT结果显示,竹荪和灵芝醇提物对MCF-7、Hela和A375细胞均有较强的抑制作用,其中,800μg/mL的竹荪醇提物对MCF-7、HeLa和A375的抑制率分别为53.46%、49.19%和61.58%(P<0.01).800μg/mL的竹荪醇提物与12.8 mg/mL竹荪灰分联用后,其对MCF-7、HeLa和A375细胞的抑制作用明显增强,对3种肿瘤细胞的抑制率分别为75.65%、69.65%和80.53%.灵芝对MCF-7、HeLa和A375细胞的抑制作用效果与竹荪一致,其中,800μg/mL的灵芝醇提物对MCF-7、HeLa和A375的抑制率分别为60.06%、54.32%和47.95%(P<0.01);800μg/mL的灵芝醇提物与12.8 mg/mL的灵芝灰分联用后对MCF-7、HeLa和A375细胞的抑制率分别增加至66.61%、65.06%和74.99%.结论 竹荪和灵芝的灰分与其抗肿瘤作用有关.
Comparative studies on the anti-tumor activity and antioxidant activity of F.velutipes and A.auricula
Objective To research and analysis with the anti-tumor activity of ashcontent of D. indusiata and G.lucidum. Methods Three human carcinoma cell lines, including MCF-7, HeLa and A375 were assessed by MTT assay to measure cell viability. Results 800 μg/mL Dictyophora alcohol extract on MCF-7, HeLa and A375 inhibition rates were 53.46%, 49.19% and 61.58% (P<0.01). When the 800 μg/mL ethanol extract and 12.8 mg/mL ash of D.indusiata on MCF-7, HeLa and A375 cells, inhibition rates increased to 75.65%, 69.65% and 80.53%. MCF-7 HeLa and A375 of G.lucidum, cells consistent with the effect of dictyophora, 800 μg/mL G.lucidum alcohol extract on MCF-7, HeLa and A375 inhibition rates were 60.06%, 54.32% and 47.95% (P<0.01). When the 800 μg/mL ethanol extract and 12.8 mg/mL ash of G.lucidumon MCF-7, HeLa and A375 cells ,inhibition rates increased to 66.61%、65.06% and 74.99%. Conclusion The ash of G.lucidum and D.indusiata has anti-tumor effect.

D.indusiataG.lucidumanti-tumor activity

佟可心、刘明月、谢明杰

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辽宁师范大学 生命科学学院,辽宁省生物技术与分子药物研发重点实验室,辽宁 大连 116081

竹荪 灵芝 抗肿瘤作用

辽宁省教育厅科学研究一般项目辽宁省自然科学基金

L201683675201602462

2017

中国生化药物杂志
南京生物化学制药研究所,全国生化制药情报中心站,中国生化制药工业协会,中国药品生物制品检定所

中国生化药物杂志

ISSN:1005-1678
年,卷(期):2017.37(11)
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