Effect of gallic acid on apoptosis of human hepatocellular carcinoma SMMC-7721 cells and its possible mechanism
Objective To investigate the effect of gallic acid on the apoptosis of human hepatocellular carcinoma SMMC-7721 cells and its possible mechanism. Methods DNA activity was measured by DNA agarose gel electrophoresis. The DNA degradation of SMMC-7721 cells was observed by flow cytometry. The apoptosis of SMMC-7721 cells was detected by flow cytometry. The expression of IC50 was detected by MTT assay. Western blot to detect the expression of related proteins. Results The IC50 of SMMC-7721 cells treated with GA was (31.2 ± 1.8) μg/mL;12.5, 50, 50 μg/mL GA for 48 h after SMMC-7721 cells, the typical DNA apoptotic bands were observed by DNA electrophoresis. GA inhibited the mitochondrial membrane potential of SMMC-7721 cells, and the peak value was significantly shifted to the left. GA activated the caspase-3 and 9 apoptotic pathway of SMMC-7721 cells to up-regulate the expression of pro-apoptotic protein Bax. Conclusion GA may induce the apoptosis of SMMC-7721 cells by mitochondrial apoptosis and inhibit the proliferation of SMMC-7721 cells.
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NSTL
万方数据
维普
