Effect and mechanism of inulicin on Alzheimer's disease model rats
Objective To discuss the effects and mechanisms of inulicin on Alzheimer's disease (AD) model rats. Methods The rat models with AD, which were built by injecting Aβ25-35, were divided into the model control group and inulicin-treated group. Besides, there were 12 healthy rats in the control group. All rats were received inulicin or saline once 1 day for 21 days. The expressions of COX-2 and iNOS in hippocampus tissue were measured by western blot and immunohistochemistry staining. Besides, the effects of inulicin on rats' learning and memory were evaluated by Morris water maze method. Results After treatment, the expressions of COX-2 and iNOS in hippocampus tissue of AD model rats were higher than the control rats(P<0.05). But the the expressions of COX-2 and iNOS in hippocampus tissue of AD model rats with inulicin were inhibited, which were lower than the model rats(P<0.05). The abnormal histomorphology of AD rats' hippocampus was improved by inulicin. Besides, inulicin improved the learning and memory capacity in the inulicin treated rats, which were with less incubation and more times of acrossing the target area than the AD model rats,(P<0.05). Conclusion There are significant evidences that inulicin could effectively inhibit the expressions of COX-2 and iNOS of AD rats' hippocampus, and decrease the inflammation, which would have a good prospect of clinical application for the treatment of Alzheimer's disease.
NETL
NSTL
万方数据
维普
