Acinetobacter baumannii and Klebsiella pneumoniae in the evaluation of tigecycline in vitro drug sensitivity test methods
Objective To evaluate the accuracy of three different detection methods for the pharmacodynamics of Acinetobacter baumannii and Klebsiella pneumoniae against tigecycline in vitro. Methods A total of 241 strains of Acinetobacter baumannii and 256 strains of Klebsiella pneumoniae were collected from Zhoushan Hospital of Zhejiang Province. Two kinds of microbes were tested by Vitek 2 method, disc diffusion method and MIC test method. Plus cyclosporine resistance, and compare the accuracy of different methods. Results The sensitivity of Acinetobacter baumannii to tigecycline was 70.2%, 78.7% and 91.5%, respectively. The Klebsiella pneumoniae sensitivity was 89.1%, 58.7% and 91.3%, respectively. Results The Vitek 2 method was more stable than that of the MIC test strip in the drug sensitivity analysis of Klebsiella pneumoniae. The results showed that the Vitek 2 method was more accurate than that of the MIC test strip But not for Acinetobacter baumannii than the paper diffusion method. Conclusion The detection of susceptibility to Acinetobacter baumannii was highly accurate by the disc diffusion method, and the detection of Vitek 2 was more accurate for Klebsiella pneumoniae.