PCR检验法与细菌培养法应用于阴道细菌检验中的价值分析
Analysis on the value of PCR test and bacterial culture method in vaginal bacteria test
李伟萍 1钟旭军1
作者信息
- 1. 杭州市富阳区第二人民医院 检验科,浙江 杭州 311404
- 折叠
摘要
目的 分析阴道细菌检验中应用PCR检验法与细菌培养法的临床价值.方法 选取2015年5月~2017年5月杭州市富阳区第二人民医院治疗的128例细菌性阴道炎患者临床资料,按检验方式不同分观察组和对照组,每组64例,对照组行细菌培养法予以测定,观察组行PCR检测法予以测定.比较2组细菌阳性、阴性检出率及棒状杆菌、加特纳菌、肠球菌阳性检出率情况.结果 观察组细菌阳性检出率比对照组高,阴性检出率比对照组低(P<0.05),观察组棒状杆菌、加特纳菌、肠球菌检出率均比对照组高(P<0.05).结论 细菌培养法、PCR检验法均可检验出阴道分泌物中细菌,但相比于细菌培养法,PCR检验法在阴道细菌检验中更具显著价值.
Abstract
Objective To analyze the clinical value of PCR test and bacterial culture in vaginal bacteria test. Methods The clinical data of 128 patients with bacterial vaginitis treated in the second people 's hospital in Fuyang district from May 2015 to May 2017 were divided into 2 groups according to the test method. The control group were tested by bacterial culture, the observation group were tested by PCR. The detection rate of positive and negative bacteria and the positive rate of Corynebacterium, Gardnerella and Enterococcus were compared between the two groups. Results The bacteria positive rate in the observation group was higher than that in the control group, the negative rate was lower than that in the control group (P<0.05, P<0.01), the Corynebacterium, Gardnerella vaginalis, enterococci detection rate in the observation group was higher than that in the control group (P<0.05). Conclusion The bacteria in vaginal secretions could be tested by bacterial culture and PCR test. Compared with bacterial culture, PCR test is more valuable than vaginal bacteria test.
关键词
细菌性阴道炎/阴道细菌/细菌培养法/PCR检验法Key words
bacterial vaginitis/vaginal bacteria/bacterial culture/PCR test引用本文复制引用
出版年
2017
NETL
NSTL
维普
万方数据