Enzymatic hydrolysis of lotus leaves to prepare small peptides
Using lotus leaf as raw material,small molecular polypeptides were prepared by enzymatic hydrolysis of lotus leaf assisted by alkaline protease.The four factors of substrate concentration,enzymolysis temperature,enzymolysis pH and enzymolysis time were tested by single factor test.The extraction process of polypeptide from lotus leaf was optimized by response surface test afterwards.Finally,the optimum conditions for enzymatic hydrolysis of lotus leaf polypeptides were as follows:substrate concentration 4.07％,enzymatic hydrolysis temperature 54.96 ℃,enzymatic hydrolysis pH 8.88,and enzymatic hydrolysis time 5.19 h.Under the above conditions,the concentration of polypeptide was 8.98×10-3 mg/mL.
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万方数据
维普
