Ultrasonic-assisted high freezing point deep eutectic solvent extraction of flavonoids from Astragalus membranaceus
In order to apply the new green solvent to the extraction of active ingredients of traditional Chinese medicine and improve the limiting factors of deep eutectic solvent in the extraction field,ultrasound-assisted deep eutectic solvent was used to extract five flavonoids in Astragalus membranaceus.The best one for the extraction of five flavonoids from Astragalus membranaceus was selected from ten different deep eutectic solvents.The effects of ultrasonic temperature,ultrasonic time,liquid-solid ratio,water content and ultrasonic power on the yield of five flavonoids were investigated by single factor experiment.The results showed that the deep eutectic solvents choline chloride-oxalic acid(1∶2)was the best extraction solvent.The optimal conditions were:ultrasonic time 43 min,ultrasonic temperature 44 ℃,and water content 54％.In this scenario,the total yield of five flavonoids reached 1.70 mg/g,which was higher than those obtained using conventional organic solvents.Therefore,ultrasonic-assisted deep eutectic solvents extraction could effectively improve the yield of Astragalus flavonoids.
deep eutectic solventsAstragalus membranaceusflavonoidsresponse surface methodologyultrasonic-assisted
NETL
NSTL
万方数据
维普
