Effects of microbial enzyme complex on growth performance,nutrient apparent digestibility,intestinal digestive enzyme activity and cecal microflora of laying hens fed wheat-based diet
In order to explore the effect of microbial enzyme complex in wheat-based laying hens diet,360 Jinghong commercial laying hens were randomly divided into three treatment groups:corn-soybean diet group(control group),corn-wheat-soybean diet group(wheat group,replace 10％corn with 10％wheat),corn-wheat-soybean-microbial enzyme complex group(microbial enzyme complex group,0.02％microbial enzyme complex was added to wheat-based laying hens diet).The results showed that the average egg weight,egg production rate,average daily feed intake and the ratio of feed to egg were not affected by different treatments.Compared with control group,the mortality of laying hens in wheat group increased by 9.0％,and the rate of broken defective eggs increased by 2.9％(P＞0.05).Compared with wheat group,the ratio of feed to egg treated with microbial enzyme complex decreased by 3.4％,the mortality decreased by 9.8％,and the rate of broken defective eggs decreased by 1.0％(P＞0.05).The treatment of wheat didn't affect the nutrient apparent digestibility(P＞0.05).Compared with wheat group,the apparent digestibility of dry matter and calcium treated with microbial enzyme complex increased by 3.9％and 5.8％respectively(P＞0.05),and the apparent digestibility of crude protein and phosphorus increased by 8.4％and 11.1％respectively(P＜0.05).The treatment of wheat didn't affect the activities of amylase,lipase and protease in duodenum and jejunum(P＞0.05).Compared with wheat treatment,the amylase activity in treatment of microbial enzyme complex increased by 15.5％(duodenum)and 24.8％(jejunum),the lipase activity increased by 22.6％(duodenum)and 23.3％(jejunum),and the protease activity increased by 9.3％(duodenum)and 8.8％(jejunum)(P＜0.05).The treatment of wheat didn't affect Escherichia coli,Salmonella and Lactobacillus in cecum(P＞0.05).Compared with wheat treatment,the treatment of microbial enzyme complex reduced the number of Escherichia coli and Salmonella in cecum by 15.5％and 13.4％respectively,and the number of Lactobacillus increased by 12.7％(P＜0.05).In conclusion,the replacement of 10％corn with 10％wheat did't cause negative performance for laying hens.The addition of microbial enzyme complex could improve the nutrient apparent digestibility,intestinal digestive enzyme activity,intestinal microbial composition and growth performance of laying hens fed with wheat-based diet.
NETL
NSTL
万方数据
维普
