Effects of dietary rutin on body size indices,rumen fermentation and organ development of Nubian goats
This experiment was conducted to investigate the effects of dietary rutin supplementation on body size indexes,rumen fermentation and organ development of Nubian goats.Thirty-six 2-month-old healthy Nubian goat ewes with similar body weight were randomly divided into 3 groups with 12 goats per group.The hens were fed basal diet(control group),basal diet+25 mg/kg rutin(trial group Ⅰ)and basal diet+50 mg/kg rutin(trial group Ⅱ),respectively.The experimental pre-feeding period lasted for 10 days and the experimental period lasted for 60 days.Slaughter was carried out after feeding.The results show that:(1)Compared with the control group,the tube circumference of test groups Ⅰ and Ⅱ on the 10th day of trial period was significantly increased by 5.57％(P＜0.05)and 6.93％(P＜0.05),respectively,and the tube circumference and body height of test group Ⅱ on the 60th day of trial period were significantly decreased by 6.54％(P＜0.05)and 8.23％(P＜0.05),respectively.The body length of trial group Ⅱ was significantly increased by 3.69％compared with that of group Ⅰ(P＜0.05).(2)Compared with the control group,the concentrations of propionic acid and total volatile fatty acid of goats in trial group Ⅰ were significantly decreased by 42.14％(P＜0.05)and 21.62％(P＜0.05),respectively.Acetic acid and total volatile fatty acid concentrations in trial group Ⅱ were significantly decreased by 29.23％(P＜0.05)and 29.43％(P＜0.05),respectively.(3)Compared with the control group,the gene relatire expression of Fibrobacter succinogenes,Butyrivibrio fibrisolvens,Lactobacillus sPP and Ruminococcus in the rumen of goat of trial group Ⅰ was increased by 271.57％(P＜0.01),1686.09％(P＜0.05),280.00％(P＜0.01)and 16226.73％(P＜0.01),respectively.The gene relatire expression of Ruminococcus albus,Butyrivibrio fibrisolvens,Prevotella ruminicola,Lactobacillus and Ruminococcus flavefaciens increased by 130.56％(P＜0.01),381.74％(P＜0.05),188.00％(P＜0.01),546.00％(P＜0.01),25624.75％(P＜0.01),respectively.The relative expression level of Ruminobacter amylophilus gene was decreased by 57.89％(P＜0.05).Trial group Ⅰ and trial group n Ruminobacter amylophilus,Fibrobacter succinogenes,Ruminococcus albus,Butyrivibrio fibrisolvens,Prevotella ruminicola and Lactobacillus sPP gene relative expression was significantly different(P＜0.01)or significant(P＜0.05),while Ruminococcus flavefaciens gene relative expression was not significantly different(P＞0.05).(4)Compared with the control group,the heart weight,omasum weight and index of goats in trialⅠ group were significantly decreased by 14.50％(P＜0.05),24.00％(P＜0.05)and 20.00％(P＜0.05),respectively.The liver weight and index of trial group Ⅱ were increased by 12.54％(P＜0.01)and 14.95％(P＜0.01),respectively,and the omasum weight and index were significantly decreased by 29.41％(P＜0.05)and 28.57％(P＜0.05),respectively.(5)Compared with the control group,duodenal VH/CD and ileum VH in trial group Ⅰ were significantly decreased by 0.13％(P＜0.05)and 15.42％(P＜0.05),respectively.Duodenal VH,CD,ileum VH and VH/CD in trial group Ⅱ were significantly decreased by 29.19％(P＜0.05),20.92％(P＜0.05),18.23％(P＜0.05)and 26.14％(P＜0.05),respectively,and jejunal CD was significantly increased by 15.63％(P＜0.05).In conclusion,under the conditions of this experiment,dietary addition of high-dose rutin could increase the number of rumen microflora of some Nubian goats,affect rumen fermentation,and had a negative effect on gastrointestinal development.
rutingoatbody rulerrumen fermentationbacterial floraorgan weightintestinal development
NETL
NSTL
万方数据
维普
