This experiment was conducted to investigate the effects of dietary rutin supplementation on body size indexes,rumen fermentation and organ development of Nubian goats.Thirty-six 2-month-old healthy Nubian goat ewes with similar body weight were randomly divided into 3 groups with 12 goats per group.The hens were fed basal diet(control group),basal diet+25 mg/kg rutin(trial group Ⅰ)and basal diet+50 mg/kg rutin(trial group Ⅱ),respectively.The experimental pre-feeding period lasted for 10 days and the experimental period lasted for 60 days.Slaughter was carried out after feeding.The results show that:(1)Compared with the control group,the tube circumference of test groups Ⅰ and Ⅱ on the 10th day of trial period was significantly increased by 5.57％(P＜0.05)and 6.93％(P＜0.05),respectively,and the tube circumference and body height of test group Ⅱ on the 60th day of trial period were significantly decreased by 6.54％(P＜0.05)and 8.23％(P＜0.05),respectively.The body length of trial group Ⅱ was significantly increased by 3.69％compared with that of group Ⅰ(P＜0.05).(2)Compared with the control group,the concentrations of propionic acid and total volatile fatty acid of goats in trial group Ⅰ were significantly decreased by 42.14％(P＜0.05)and 21.62％(P＜0.05),respectively.Acetic acid and total volatile fatty acid concentrations in trial group Ⅱ were significantly decreased by 29.23％(P＜0.05)and 29.43％(P＜0.05),respectively.(3)Compared with the control group,the gene relatire expression of Fibrobacter succinogenes,Butyrivibrio fibrisolvens,Lactobacillus sPP and Ruminococcus in the rumen of goat of trial group Ⅰ was increased by 271.57％(P＜0.01),1686.09％(P＜0.05),280.00％(P＜0.01)and 16226.73％(P＜0.01),respectively.The gene relatire expression of Ruminococcus albus,Butyrivibrio fibrisolvens,Prevotella ruminicola,Lactobacillus and Ruminococcus flavefaciens increased by 130.56％(P＜0.01),381.74％(P＜0.05),188.00％(P＜0.01),546.00％(P＜0.01),25624.75％(P＜0.01),respectively.The relative expression level of Ruminobacter amylophilus gene was decreased by 57.89％(P＜0.05).Trial group Ⅰ and trial group n Ruminobacter amylophilus,Fibrobacter succinogenes,Ruminococcus albus,Butyrivibrio fibrisolvens,Prevotella ruminicola and Lactobacillus sPP gene relative expression was significantly different(P＜0.01)or significant(P＜0.05),while Ruminococcus flavefaciens gene relative expression was not significantly different(P＞0.05).(4)Compared with the control group,the heart weight,omasum weight and index of goats in trialⅠ group were significantly decreased by 14.50％(P＜0.05),24.00％(P＜0.05)and 20.00％(P＜0.05),respectively.The liver weight and index of trial group Ⅱ were increased by 12.54％(P＜0.01)and 14.95％(P＜0.01),respectively,and the omasum weight and index were significantly decreased by 29.41％(P＜0.05)and 28.57％(P＜0.05),respectively.(5)Compared with the control group,duodenal VH/CD and ileum VH in trial group Ⅰ were significantly decreased by 0.13％(P＜0.05)and 15.42％(P＜0.05),respectively.Duodenal VH,CD,ileum VH and VH/CD in trial group Ⅱ were significantly decreased by 29.19％(P＜0.05),20.92％(P＜0.05),18.23％(P＜0.05)and 26.14％(P＜0.05),respectively,and jejunal CD was significantly increased by 15.63％(P＜0.05).In conclusion,under the conditions of this experiment,dietary addition of high-dose rutin could increase the number of rumen microflora of some Nubian goats,affect rumen fermentation,and had a negative effect on gastrointestinal development.
rutingoatbody rulerrumen fermentationbacterial floraorgan weightintestinal development
NETL
NSTL
维普
万方数据
