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不同乳酸菌固态发酵对黑水虻幼虫营养价值的影响

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本试验旨在探讨不同乳酸菌固态发酵对黑水虻幼虫中主要营养成分、pH、乳酸和多肽含量的影响,试验分为4组,分别为对照组(未发酵)、试验1组(植物乳杆菌组)、试验2组(粪肠球菌组)、试验3组(嗜酸乳杆菌组),每组3个平行.将黑水虻幼虫与麸皮按1∶1混合,分别添加发酵用种子液,将混匀后的样品分别装入发酵袋中,放于35℃人工气候箱中发酵3 d.结果 表明:与对照组相比,试验1组、2组、3组粗纤维含量均显著下降(P<0.05);缬氨酸含量分别增加了 11.25%(P<0.05)、10.00%(P<0.05)和 16.25%(P<0.05),脯氨酸含量分别增加了 15.73%(P<0.05)、13.75%(P<0.05)和8.99%(P<0.05);各组之间乳酸含量差异极显著(P<0.01),其中试验1组乳酸含量最高,达到了 8.72%;发酵后,各试验组pH显著下降(P<0.01),其中试验1组pH最低;发酵后3个试验组多肽含量均显著升高(P<0.01).综合考虑,优先选择植物乳杆菌作为黑水虻发酵饲料发酵剂.
Effects of solid-state fermentation of different lactic acid bacteria on the nutritional value of black soldier fly
In order to investigate the effects of solid-state fermentation of different lactic acid bacteria on the nutritional index,pH,lactic acid and peptide content of the black soldier fly larvae.The experiment included 4 groups,namely control group(unfermented),test group 1(Lactobacillus plantarum),test group 2(Enterococcus faecalis)and test group 3(Lactobacillus acidophilus),with three replicates per group.Black soldier fly larvae were mixed with bran at 1∶1,seed solution for fermentation was added separately.The mixed samples were put into fermentation bags separately and placed in an artificial climate chamber at 35℃ for 3 days.The results showed that three Lactobacillus fermentation starters could significantly reduce the crude fibre content(P<0.05)compared with the control group.The valine content increased by 11.25%(P<0.05),10.00%(P<0.05)and 16.25%(P<0.05)respectively,and the proline content increased by 15.73%(P<0.01),13.75%(P<0.01).Lactic acid content was significantly different among all groups(P<0.01),and lactic acid content in test group 1 was the highest,reaching 8.72%.The pH of each test group decreased significantly after fermentation(P<0.01),with the lowest pH in test group 1.The polypeptide content increased significantly in all three test groups after fermentation(P<0.01).In conclusion,Lactobacillus plantarum was preferred as a feed starter for black water fly fermentation.

lactic acid bacteriablack soldier fly larvaesolid-state fermentationnutritional ingredient

郭建来、和月、魏红芳、刘昆、姬向波、翟乐平

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河南牧业经济学院河南省非常规饲料资源创新利用重点实验室,河南郑州 450045

河南牧业经济学院动物科技学院,河南郑州 450045

河南同发生物科技有限公司,河南郑州 450000

乳酸菌 黑水虻幼虫 固态发酵 营养成分

河南省高等学校重点科研项目河南省科技攻关项目

22B230003222102110390

2024

中国饲料
中国饲料工业协会

中国饲料

北大核心
影响因子:0.577
ISSN:1004-3314
年,卷(期):2024.(1)
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