Determination of vitamin B1 and vitamin B2 in feed by HPLC with fluorescence
To establish an analytical method for the determination of vitamin B1 and vitamin B2 in feed by high performance liquid chromatography-fluorescence,the feed was pretreated by high temperature and high pressure method,and the extract was derivatized.Vitamin B1 and vitamin B2 were determined at different wavelengths with sodium acetate-methanol as mobile phase.The results showed that the liner range were 0.03～5.00 μg/mL.The correlation coefficients were greater than 0.9999.The recoveries were 92.7％～98.1％.The limit of detection respective were 0.04 mg/kg and 0.06 mg/kg of vitamin B,ang vitamin B2.The limit of quantitation respective were 0.15 mg/kg and 0.18 mg/kg of vitamin B1 ang vitamin B2.This method was convenient and accurate for the determination of vitamin B,and B2 in feed.
high performance liquid chromatography-fluorescencefeedvitamin B1 and vitamin B2
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