To establish an analytical method for the determination of vitamin B1 and vitamin B2 in feed by high performance liquid chromatography-fluorescence,the feed was pretreated by high temperature and high pressure method,and the extract was derivatized.Vitamin B1 and vitamin B2 were determined at different wavelengths with sodium acetate-methanol as mobile phase.The results showed that the liner range were 0.03～5.00 μg/mL.The correlation coefficients were greater than 0.9999.The recoveries were 92.7％～98.1％.The limit of detection respective were 0.04 mg/kg and 0.06 mg/kg of vitamin B,ang vitamin B2.The limit of quantitation respective were 0.15 mg/kg and 0.18 mg/kg of vitamin B1 ang vitamin B2.This method was convenient and accurate for the determination of vitamin B,and B2 in feed.
high performance liquid chromatography-fluorescencefeedvitamin B1 and vitamin B2
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