This study was based on the high-performan ce liquid chromatography-tandem mass spectrometry(UPLC-MS)combined with QuEChERS rapid purification method,a method was developed for the quantitative analysis of sudan,rhodamine B and acid orange Ⅱ in poultry feed.The samples were extracted with acetonitrile and purified with C18+PSA.The purified liquid was separated by Hypersil GOLD C18 reversed-phase chromatography column,multi-reaction monitoring(MRM)was performed in an electrospray ionization source(ESI)with positive and negative ion double-scan mode,the quantity was determined by external standard curve method.The results showed that the correlation coefficient(R2)＞0.999,the detection limits(LOD)of sudan and acid orange Ⅱ were 0.5 μg/kg and quantitative limits(LOQ)were 1.0 μg/kg,the LOD of rhodamine B were 0.05 μg/kg and the LOQ were 0.1 μg/kg.The recoveries ranged from 90％～110％,RSD＜10％.This study had the advantages of fast and sensitive,it had realized the accurate quantitative analysis of 6 chemical dyes in poultry feed,and provided technical reference for the quality and safety of poultry feed.
关键词
禽用饲料/苏丹红/罗丹明B/酸性橙Ⅱ/超高效液相色谱-串联质谱法/QuEChERS
Key words
poultry feed/sudan/rhodamine B/acid orange Ⅱ/high-performance liquid chromatography-tandem mass spectrometry/QuEChERS
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