This experiment was conducted to investigate the effects of supplementary feeding fermented soybean meal on intestinal microflora diversity and microflora structure composition of Yili mares.Fifteen 12-month-old Yili foals with similar body condition and no history of digestive diseases were randomly divided into three groups with five horses in each group.Control group,experimental groups Ⅰ and Ⅱ were added with 5％common soybean meal,2.5％common soybean meal+2.5％fermented soybean meal and 5％fermented soybean meal on the basis of hay and ordinary concentrate diet,respectively.Prefeeding lasted for 7 days,and the experiment lasted for 56 days.The results showed as follows:A total of 2672029 NO.effective sequences and 32234 amplicon sequence patterns(ASVs)were acquired;With the increase of foal age,the abundance of helicoid in test group Ⅰ was significantly lower on day 56 than on day 0(P＜0.01);Proteobacteria in test group Ⅱwere significantly increased(P＜0.05),and actinobacteria abundance was significantly decreased(P＜0.05);With the increase of fermented soybean meal supplemental level,the abundance of ruminococcus family in test group Ⅱ was significantly higher than that in control group(P＜0.05),and the abundance of Streptococcus family was significantly lower than that in control group(P＜0.05).In conclusion,supplementation with fermented soybean meal could effectively improve the digestive capacity of foals,prevent the occurrence of hoof inflammation,diarrhea and inflammation,and promote the stability of intestinal flora of foals.
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