响应面法优化柞蚕蛹虫草活性肽提取工艺研究
Optimization of extraction process of active peptide from Cordyceps militaris by response surface method
刘博佳 1李正钰 2柳叶飞 3王泽 4徐方旭5
作者信息
- 1. 沈阳师范大学生命科学学院,辽宁沈阳 110034
- 2. 沈阳师范大学粮食学院,辽宁沈阳 110034
- 3. 沈阳师范大学实验教学中心,辽宁沈阳 110034;辽宁省功能性蛹虫草重点实验室,辽宁沈阳 110034;辽宁省蛹虫草种质资源库,辽宁沈阳 110034
- 4. 沈阳师范大学生命科学学院,辽宁沈阳 110034;沈阳市功能性蛹虫草产业技术研究院,辽宁沈阳 110034
- 5. 沈阳师范大学实验教学中心,辽宁沈阳 110034;辽宁省功能性蛹虫草重点实验室,辽宁沈阳 110034
- 折叠
摘要
为优化柞蚕蛹虫草多肽提取工艺,本试验分别研究了 pH、酶解时间、反应温度和加酶量对多肽得率的影响,并采用响应面法对提取条件进行了优化.结果表明:四种因素对柞蚕蛹虫草多肽得率的影响顺序为pH＞酶解时间＞反应温度＞加酶量,最佳酶解条件为:酶解时间2.8 h、加酶量2％、pH 9.7、反应温度56 ℃.在此酶解条件下进行验证试验,测得柞蚕蛹虫草多肽得率平均值为86.1％,与预测值87.52％接近,表明该模型能较好地预测辅助提取柞蚕蛹虫草活性肽,可为柞蚕蛹虫草的产业性开发应用提供依据.
Abstract
In order to optimize the extraction process of polypeptide from tussah silkworm pupa,the effects of pH,enzymatic hydrolysis time,reaction temperature and enzyme dosage were studied,and the extraction conditions were optimized by response surface method.The results showed that the effect order of four factors on the polypeptide yield of Cordyceps militaris cultivated with tussah pupa was pH＞enzymatic hydrolysis time＞reaction temperature＞enzyme dosage.The optimum enzymolysis conditions were as follows:enzymolysis time 2.8 h,enzyme dosage 2％,pH 9.7,reaction temperature 56 ℃.The experiment was repeated three times under the condition of enzymolysis.The average yield of polypeptide was 86.1％,which was close to the predicted value of 87.52％,indicating that the model could well predict the assisted extraction of active peptide of Cordyceps militaris,and could provide a basis for the industrial development and application of Cordyceps militaris cultivated with tussah pupa.
关键词
柞蚕蛹虫草/活性肽/响应面法Key words
Cordyceps militaris cultivated with tussah pupa/active peptide/response surface methodology引用本文复制引用
基金项目
辽宁省教育厅重点攻关项目(LJKZZ20220116)
辽宁省科技厅面上项目(2023-MS-251)
省级大学生创新创业训练计划资助项目(S202310166019)
出版年
2024
国家科技期刊平台
NSTL
万方数据