Simultaneous determination of olaquindox,chloramphenicol and nitrofuran residues in fishery non-pharmaceutical inputs by ultra performance liquid chromatography tandem mass spectrometry
An ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)method was developed for the simultaneous determination of olaquindox,chloramphenicol and nitrofuran residues in fishery non-pharmaceutical inputs.Acetonitrile was used as the extraction agent,and eight drugs in the input were extracted by vortex ultrasound in a dark environment.The solid phase extraction column was used to purify the extract and analyze the instrument.The liquid chromatography was carried out with water and acetonitrile as the flow.The BEH C18 column was used to separate the drugs,and the mass spectrometry was scanned simultaneously in the positive and negative ion switching mode.The olaquindox and nitrofuran drugs were quantified by matrix-matched external standard method,and the chloramphenicol drugs were quantified by matrix-matched internal standard method with deuterated chloramphenicol as internal standard.The results showed that the linear range of olaquindox and nitrofurans was 0.5～20.0 ng/mL,and the linear range of chloramphenicol was 0.2～20.0 ng/mL.The correlation coefficient of the standard curve was 0.9987～0.9996.The limit of detection of the eight drugs was 1.0～2.5 μg/kg,and the limit of quantitation was 2.5～5.0 µg/kg.The recoveries were 75.5％～102.2％,and the relative standard deviations were 2.8％～10.2％.This method could be used to extract and determine 8 kinds of drugs at the same time.The detection efficiency was high,and the accuracy and precision of the method were good.It could be used for the determination of 8 drug residues in fishery non-pharmaceutical inputs.
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