Research on construction of rapid propagation system for Cyperus esculentus
To construct the rapid propagation system for Cyperus esculentus,Heiyousha No.2 was taken the test material with its stem tip as explant to obtain the aseptic seedling of Cyperus esculentus through plant tissue culture technology,as well as screen the best sterilization method for stem tip,appropriate differential medium formula for stem tip and rapid rooting medium formula.According to the results,the contamination rate for stem tip of Heiyousha No.2 shows a reducing trend along with increasing sterilization time,and the survival rate showed a change from rising to descending;If carrying out MS+0.9 mg/L 6-benzyl adenine(6-BA)+0.3 mg/L 1-naphthaleneacetic acid(NAA)treatment,the seedling number differentiated from stem tip of Heiyousha No.2 was obviously higher than other treatment(P＜0.05),and the seeding percentage differentiated from stem tip was the highest,which is 62.67％.If carrying out 1/2MS+0.2 mg/L Indole-3-butyric acid(IBA)treatment,the rooting number for aseptic seedling of Heiyousha No.2 was the highest(29.00),which was obviously higher than other treatment(P＜0.05),and the root was thick and strong with the root length was 0.9～3.0 cm.In summary,75％ethyl alcohol 30 s+0.1％L HgCl2 4 min were the best sterilization method for stem tip of Heiyousha No.2,and MS+0.9 mg/L 6-BA+0.3 mg/L NAA was the best differential medium formula for stem tip,MS+1.0 mg/L 6-BA and 1/2MS+0.2 mg/L IBA were the best rapid propagation and rooting medium formulations,respectively.The construction of rapid propagation system for Cyperus esculentus provide a basis for the high-efficiency propagation and breeding of excellent seedings of Cyperus esculentus and germplasm resources innovation in cold regions.
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