Optimization of culture conditions of Saccharomyces cerevisiae
The culture conditions of Saccharomyces cerevisiae were optimized by single factor test and response surface methodology.First,the important conditions of dry mass culture of Saccharomyces cerevisiae were used for single factor test,and 4 important influencing factors such as culture temperature,culture time,initial pH of medium,and bacterial age were screened,and the response surface experiment was designed according to the Box-Behnken design principle.Then the multiple quadratic regression equation with the dry cell mass of yeast as the response value was established.The analysis model showed that the optimal culture conditions of Saccharomyces cerevisiae were as follows:culture temperature 23.5℃,culture time 71.5 h,initial pH of medium 6.5,Bacteria age 12 h.Under the optimal culture conditions,the dry mass of yeast cells reached(29.70±0.04)g/L,which was 31.39%higher than that before optimization.
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