Effects of feeding compound probiotics on fecal microbial diversity of Hu sheep
This experiment was conducted to study the effect of compound probiotics on the fecal microbial diversity of Hu sheep.40 Hu sheep of similar age and weight(34.6±0.65)kg were selected and randomly divided into control group(CG group)and probiotic group(EG group)according to the principle of"similar weight",with 20 sheep in each group.CG group and EG group were fed with total mixed diet respectively,total mixed diet and compound probiotics.The test period was 60 days.10 Hu sheep were randomly selected for each treatment,and fecal samples were collected at the end of the rectum.16S rDNA technology was used for sequencing analysis.The results showed that:(1)Feeding compound probiotics could significantly reduce the diarrhea rate of Hu sheep(P＜0.05).(2)According to OTU cluster analysis,the OTUs of the EG group(599)was higher than that of CG group(466),and the Ace index,Chao 1 index and Shannon index of the EG group were significantly higher than that of CG group(P＜0.05).The Simpson index was not significantly different between two groups(P＞0.05).(3)At the phylum level,the relative abundance of Firmicutes,Bacteroidota,Spirochaetota,Verrucomicrobiota and fibrobacterota in EG group was higher than that in CG group,and that of Proteobacteria was lower than that in CG group,but the difference was not significant(P＞0.05);At the genus level,the two groups of dominant bacteria were UCG_005,unclassified_Lachnospiraceae,Rikenellaceae_RC9_gut_group and Bacteroidetes,EG group was higher than CG group,but the difference was not significant(P＞0.05).(4)The LEfSe analysis results showed that there were 7 probiotic groups with significant differences compared with CG group,mainly Oscillospirales and Rikenellaceae.In conclusion,under the conditions of this experiment,feeding compound probiotics could effectively improve the structure of intestinal microbial community of Hu sheep,and promote the metabolic function of intestinal microorganisms.
万方数据
维普
